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| 植物药材总DNA提取 | |
| 引用本文： | 李晓波,冯波,张朝晖,王峥涛,徐珞珊.植物药材总DNA提取[J].中草药,2002,33(7):652-654. |
| 作者姓名： | 李晓波 冯波 张朝晖 王峥涛 徐珞珊 |
| 作者单位： | 1. 上海交通大学药学院,上海,200030;中国药科大学中药学院,江苏,南京,210038 2. 中国药科大学中药学院,江苏,南京,210038 3. 第二军医大学南京军医学院,江苏,南京,210099 |
| 基金项目： | 中国博士后科学基金 (1998年 ) |
| 摘 要： | 目的 为获得高质量的DNA，研究木质部较发达的根，根茎类以及茎木类药材的DNA提取方法。方法 总DNA的提取过程包括用Tris缓冲液洗净，CTAB抽提和纯化。结果 对升高，Chong木，木香，乌药，芍药和木通等进行了DNA提取，能较好的去除色素，多糖等干扰PCR物质。结论 本法使用常用试剂，成本低，易于推广，为药材DNA分子鉴别的应用奠定了基础。 |
| 关 键 词： | 植物药材 DNA提取 NDA分子鉴别 PCR |
| 文章编号： | 0253-2670(2002)07-0652-03 |
| 收稿时间： | 2001/11/6 0:00:00 |
| 修稿时间： | 2001年11月6日 |
Isolation of total DNA from Plant Chinese medicinal materials |
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| Li Xiaobo,Feng Bo,Zhang Chaohui,Wang Zhengtao and Xu Luoshan.Isolation of total DNA from Plant Chinese medicinal materials[J].Chinese Traditional and Herbal Drugs,2002,33(7):652-654. | |
| Authors: | Li Xiaobo Feng Bo Zhang Chaohui Wang Zhengtao and Xu Luoshan |
| Institution: | LI Xiao bo 1,2,FENG Bo 2,ZHANG Zhao hui 3,WANG Zheng tao 2,XU Luo shan 2 |
| Abstract: | Object To explore the method for high quality DNA extract from plant Chinese medicinal materials containing developed xylem Methods The process of DNA includes wash with Tris buffer, extract with CYAB buffer and purification mainly Results Using this method, the total DNA was isolated from Cimicifugae foelida L., Arlia chinensis L., Paeoniae lactiflora Pall.,Aucklandia lappa Decne.,Lindera aggregata (Slms) Kostern., and Akebia quinata Decne. etc This method is preferable in removing pigments, polysaccharides, substance disturbing PCR Conclusion In this process, common reagents are used, lower cost is of advantage to popularize This study provids the applying foundations for the DNA identification of plant Chinese medicinal materials |
| Keywords: | DNA extract plant Chinese medicinal materials Cimicifugae foelida L |
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