五指毛桃组织培养获得再生植株的研究

目的采用组织培养快繁技术培养五指毛桃种苗,为人工种植提供种源。方法采用五指毛桃叶片作外植体,以MS和1/2 MS为基本培养基,采用正交设计研究植物生长调节剂多因素组合(6-BA、NAA、2,4-D、IAA、KT和IBA)对五指毛桃初代诱导、不定芽分化和诱导生根的影响。结果不定芽最佳诱导培养基为MS+1.0 mg/L 6-BA+0.3 mg/L NAA,外植体经20 d诱导培养,可分化形成不定芽72个;MS+1.0 mg/L 6-BA+0.3 mg/L IAA+0.3 mg/L KT最利于不定芽继代增殖,增殖倍数6.67;1/2 MS+1.0 mg/L IBA+0.3 mg/L NAA最适于诱导生根获得再生植株,生根率100%;宜移栽于泥炭-珍珠岩(1︰1)的基质上,成活率为93%。结论此途径繁殖速度快、再生率高,能为栽培五指毛桃提供大量种苗。

Research on tissue culture and plant regeneration for roots of Ficus hirta

Objective To solve the shortage problem of large-scale planting the seedlings in the roots of Ficus hirta by rapid propagation in vitro tissue culture. Methods The MS and 1/2 MS media were used as basic media, The multi factor combination of plant growth regulator (6-BA, NAA, 2, 4-D, IAA, KT, and IBA) on the seedling subculture and root culture was studied by orthogonal design. Results The best medium for the adventitious bud induction was MS + 6-BA 1.0 mg/L + NAA 0.3 mg/L, 72 adventitious buds were obtained by differentiation with 20 d induction of explants; The best medium for cluster inducing and subculture was MS + 1.0 mg/L 6-BA + 0.3 mg/L IAA + 0.3 mg/L KT; The best rooting medium was 1/2 MS + 1.0 mg/L IBA + 0.3 mg/L NAA, and the rooting rate was 100%. Being suitably Transplanted to peat-perlite (1:1) matrix, the 30 d survival rate was 93%. Conclusion The tissue culture for the roots of F. hirta could be used to produce test-tube seedlings for large scale planting.