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| 鲤鱼精巢DNA对自由基的清除作用 | |
| 引用本文： | 周永红,唐孝礼,颜光美,许实波. 鲤鱼精巢DNA对自由基的清除作用[J]. 中草药, 2000, 31(12): 923-925 |
| 作者姓名： | 周永红 唐孝礼 颜光美 许实波 |
| 作者单位： | 1. 广东药学院预防医学系,广州,510224 2. 中山医科大学药理学教研室 3. 中山大学生命科学学院药学系 |
| 摘 要： | 目的：研究鲤鱼精巢DNA对自由基的清除作用。方法：采用 化学发光法测定鲤鱼精巢DNA对Cu2 -Vit C-H2O2发光体系产生的·OH自由 基的清 除作用，采用分光光度法检测鲤鱼精巢DNA对大鼠腹腔多形核白细胞(PMNs)呼吸爆发产生O ÷2自由基的清除作用，采用电子自旋共振(ESR)法测定鲤鱼精巢DNA对DPPH有机自由基的 清 除作用。结果：鲤鱼精巢DNA使Cu2 -Vit C-H2O2发光体系的发光计数率降低， 使 PMNs呼吸爆发产生O÷2自由基的数量减少，使DPPH的特征波峰逐渐降低直至消失。结论 ：鲤鱼精巢DNA对·OH自由基、O÷2自由基和有机自由基均有显著的消除作用。 |
| 关 键 词： | 鲤鱼 精巢 自由基清除 脱氧核糖核酸 动物药 |
| 收稿时间： | 2000-02-20 |
Scavenging Action of DNA from Carp Spermary on Free Radicals |
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| Zhou Yonghong,Tang Xiaoli,Yan Guangmei and Xu shibo. Scavenging Action of DNA from Carp Spermary on Free Radicals[J]. Chinese Traditional and Herbal Drugs, 2000, 31(12): 923-925 | |
| Authors: | Zhou Yonghong Tang Xiaoli Yan Guangmei Xu shibo |
| Affiliation: | Guangzhou 510224 |
| Abstract: | To investigate whether DNA from carp spermary scave nges free radicals. Chemical luminometry, spectrometry and electronic spin resonanc e (ESR) methods were used to measure the scavenging action of deoxyribonucleic ac id (DNA) from carp spermary on ·OH radical produced by Cu2+-Vit C-H2O2 chemoluminescent system, O2- radical produced by the breathing burst of polymorphonuclear leukocytes (PMNs) and DPPH radical respectively. DNA from car p spermary scavenged ·OH radical produced by Cu2+-Vit C-H2O2 chemolu minescent system, O2- radical produced by the breathing burst of PMNs and DPPH radicals. It could be concluded that DNA from carp spermary scavenges free radicals. |
| Keywords: | carp spermary deoxyribonucleic acid free radic als |
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