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| HPLC法同时测定附子中6种单酯和双酯型生物碱 | |
| 引用本文： | 孙兰,周海燕,赵润怀,游畅,高超,常琪.HPLC法同时测定附子中6种单酯和双酯型生物碱[J].中草药,2009,40(1):131-134. |
| 作者姓名： | 孙兰 周海燕 赵润怀 游畅 高超 常琪 |
| 作者单位： | 1. 中国医学科学院北京协和医学院药用植物研究所,北京,100193 2. 中国医学科学院北京协和医学院药用植物研究所,北京100193;中国药材集团公司,北京102600 3. 中国药材集团公司,北京,102600 |
| 摘 要： | 目的 建立附子中单酯及双酯型生物碱成分的高效液相色谱定量方法.方法 附子干燥粉末用氨水浸润后,以异丙醇一醋酸乙酯(1:1)超声提取30 min,提取液蒸干后溶于0.01%盐酸甲醇中,经微孔滤膜滤过后,20 pL进行HPLC分析,HPLC分析采用Zorbax Extend-C18柱(250 mmX 4.6 mm,5 μm),以乙腈-40 mmol/L醋酸铵缓冲液(氨水调pH 10.0)为流动相进行梯度洗脱,体积流量1.0 mL/min,检测波长230 nm.结果 6种被测生物碱成分,包括乌头碱、次乌头碱、新乌头碱、苯甲酰乌头原碱、苯甲酰次乌头原碱和苯甲酰新乌头原碱分离良好,各成分质量浓度与峰面积在测定范围内均呈良好的线性关系(r>0.999),重现性好,6种成分的RSD(n=5)均小于1.50%;平均加样回收率(n=5)为98.20%～100.47%;最低检测限分别为0.26、0.36、0.92、0.30、0.52、0.82 ng.结论 本研究所建立的HPLC方法稳定可靠、简便易行,可用于附子类药材中单酯型和双酯型生物碱的同时定量分析,为附子药材及其炮制品的质量评价和质量控制奠定了基础. |
| 关 键 词： | 附子 乌头碱 次乌头碱 新乌头碱}苯甲酰乌头原碱 苯甲酰次乌头原碱 苯甲酰新乌头原碱 HPLC |
| 收稿时间： | 2008/4/15 0:00:00 |
Determination of six kinds of monoester-and diester-alkaloids in Radix Aconitii Lateralis Praeparata hy HPLC |
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| SUN Lan,ZHOU Hai-yan,ZHAO Run-huai,YOU Chang,GAO Chao and CHAN Gqi.Determination of six kinds of monoester-and diester-alkaloids in Radix Aconitii Lateralis Praeparata hy HPLC[J].Chinese Traditional and Herbal Drugs,2009,40(1):131-134. | |
| Authors: | SUN Lan ZHOU Hai-yan ZHAO Run-huai YOU Chang GAO Chao and CHAN Gqi |
| Institution: | Institute of Medicinal Plant Development,Chinese Academy of Medical Science and Peking Union Medical College,Beijing100094,China;Institute of Medicinal Plant Development,Chinese Academy of Medical Science and Peking Union Medical College,Beijing100094,China;China National Group Corporation of Traditional and Herbal Medicine,Beijing 102600,China;China National Group Corporation of Traditional and Herbal Medicine,Beijing 102600,China;Institute of Medicinal Plant Development,Chinese Academy of Medical Science and Peking Union Medical College,Beijing100094,China;Institute of Medicinal Plant Development,Chinese Academy of Medical Science and Peking Union Medical College,Beijing100094,China;Institute of Medicinal Plant Development,Chinese Academy of Medical Science and Peking Union Medical College,Beijing100094,China |
| Abstract: | |
| Keywords: | Radix A conitii Lateralis Praeparaa aconitine hypaconitine mesaconitine benzoylaconine benzoyl hypaconine benzoylmesaconine HPLC |
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