| 防风愈伤组织诱导及植株再生体系的建立 |
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| 引用本文： | 付航,黄涵签,王妍,慧芳,杨世海.防风愈伤组织诱导及植株再生体系的建立[J].中草药,2018,49(13):3127-3133. |
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| 作者姓名： | 付航 黄涵签 王妍 慧芳 杨世海 |
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| 作者单位： | 吉林农业大学中药材学院 |
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| 基金项目： | 甘草等7种中药饮片标准化建设（ZYBZH-Y-JL-25） |
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| 摘 要： | 目的建立防风Saposhnikovia divaricate再生体系。方法研究不同外植体、温度、培养基、光照条件对诱导防风愈伤组织的影响,采用响应面试验设计,优化愈伤组织诱导及增殖的激素配比。结果以防风的叶片为外植体,诱导愈伤组织最适培养基为MS+6-BA 0.90 mg/L+2,4-D 1.05 mg/L+KT 0.96 mg/L,25℃恒温12 h/d光照培养;最适增殖培养基为MS+6-BA 0.56 mg/L+2,4-D 0.48 mg/L+KT 0.40 mg/L,继代周期为35 d;诱导丛生芽最适培养基为MS+NAA 0.6 mg/L+6-BA 1.0 mg/L;最适生根培养基为MS+NAA 0.4 mg/L+6-BA 0.1 mg/L。结论成功诱导防风愈伤组织并建立稳定高效的植株再生体系。
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| 关 键 词： | 防风 组织培养 愈伤组织 响应面试验设计 植株再生 |
| 收稿时间： | 2018/3/15 0:00:00 |
Callus induction and establishment of plant regeneration system of Saposhnikovia divaricate |
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| FU Hang,HUANG Han-qian,WANG Yan,HUI Fang and YANG Shi-hai.Callus induction and establishment of plant regeneration system of Saposhnikovia divaricate[J].Chinese Traditional and Herbal Drugs,2018,49(13):3127-3133. |
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| Authors: | FU Hang HUANG Han-qian WANG Yan HUI Fang and YANG Shi-hai |
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| Institution: | College of Chinese Medicine Materials, Jilin Agricultural University, Changchun 130118, China,College of Chinese Medicine Materials, Jilin Agricultural University, Changchun 130118, China,College of Chinese Medicine Materials, Jilin Agricultural University, Changchun 130118, China,College of Chinese Medicine Materials, Jilin Agricultural University, Changchun 130118, China and College of Chinese Medicine Materials, Jilin Agricultural University, Changchun 130118, China |
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| Abstract: | |
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| Keywords: | Saposhnikovia divaricate(Turcz ) Schisck tissue cultures callus response surface test design plant regeneration |
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