五味子粉对D-半乳糖致小鼠衰老模型的影响

目的观察五味子粉对D-半乳糖所致小鼠衰老模型的影响。方法 72只昆明种小鼠随机分为对照组、模型组、脑康灵(0.810 g/kg)组和五味子粉高、中、低剂量(3.00、1.50、0.75 g/kg)组。小鼠颈背部sc 1.25 g/kg D-半乳糖制备衰老模型,连续40 d,于第11天开始ig给予相应剂量的药物,连续给药30 d。于第39天进行小鼠行为学避暗实验;末次给药后2h,检测小鼠脑匀浆、肝匀浆及血浆中丙二醛(MDA)水平,全血中过氧化物酶(CAT)、超氧化物歧化酶(SOD)活性及还原型谷胱甘肽(GSH)水平;光镜下观察各组小鼠脑、肝脏、胸腺、脾脏的形态变化。结果与模型组比较,各剂量五味子粉均可延长小鼠避暗潜伏期,减少小鼠明暗穿梭次数;不同程度降低小鼠脑匀浆、肝匀浆及血浆中MDA水平,升高全血中CAT、SOD活性及GSH水平;不同程度升高小鼠脾脏、胸腺和脑指数,降低肝脏指数。结论五味子粉可显著改善衰老模型小鼠的各项生化指标和组织病理状态。

Effect of Schisandrae Chinensis Fructus powder on D-galactose-induced aging model mice

Objective To investigate effect of Schisandrae Chinensis Fructus powder in D-galactose-induced aging model mice. Methods Seventy-two Kunming mice were randomly divided into normal group, model group, Naokangling group (0.810 g/kg), and low-, mid-, and high-dose (3.00, 1.50, 0.75 g/kg) Schisandrae Chinensis Fructus powder groups. Aging mice model was established by sc injection of D-galactose 1.25 g/kg at neck back once daily for 40 d. Naokangling and Schisandrae Chinensis Fructus powder were orally administrated on day 11 for 30 d. Then the learning and memory ability was assessed by step-through test on day 39. Two hours after the last administration, the contents of malonaldehyde (MDA) in brain homogenate, liver homogenate, and plasma and the activity of superoxide dismutase (SOD), glutathione (GSH), and catalase (CAT) in whole blood were detected; The morphological changes of brain, liver, thymus, and spleen in each group of mice were observed by light microscope. Results Compared with model groups, the Schisandrae Chinensis Fructus powder groups can improve the incubation period and reduce the number of times of light and dark shuttle of model mice; The Schisandrae Chinensis Fructus powder groups can reduce the level of MDA in plasma, brain, and liver homogenate, and increase the levels of CAT, SOD, and GSH in the whole blood in different degrees; It also can elevate the index of spleen, thymus, and brain, and decrease the indexes of liver in different degrees. Conclusion Schisandrae Chinensis Fructus powder can significantly improve the biochemical indexes and pathological status of aging model mice.