| 大孔吸附树脂纯化山蜡梅叶中总黄酮的研究 |
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| 引用本文： | 张亚梅,张小娟,简晖,冯育林,王跃生,杨世林.大孔吸附树脂纯化山蜡梅叶中总黄酮的研究[J].中草药,2009,40(8):1226-1229. |
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| 作者姓名： | 张亚梅 张小娟 简晖 冯育林 王跃生 杨世林 |
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| 作者单位： | 1. 中药固体制剂制造技术国家工程研究中心,江西,南昌,330006
2. 中药固体制剂制造技术国家工程研究中心,江西,南昌,330006;江西中医学院,江西,南昌,330006 |
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| 基金项目： | 国家科技支撑汁划课题,江西省卫生厅中医药科研计划课题 |
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| 摘 要： | 目的 研究大孔树脂纯化山蜡梅叶中总黄酮的工艺条件及参数.方法 采用静态吸附-解吸方法,以吸附量和解吸率为指标,筛选最佳树脂;又以总黄酮质量浓度为指标,考察了最佳树脂纯化山蜡梅叶中总黄酮的工艺参数.结果 8种树脂中,HPD400树脂对山蜡梅叶中总黄酮纯化效果较好,其总黄酮的静态吸附量达到17.77 mg/g树脂,解吸率为92.24%;动态吸附量为1.68 g/g树脂,用4倍柱体积蒸馏水、4倍柱体积30%乙醇洗脱除去杂质后,换用70%乙醇6倍柱体积洗脱,总黄酮质量分数为31.4%,总黄酮转移率为88.4%.结论 HPD400型大孔树脂在所确定的工艺条件下,可较好地纯化山蜡梅叶总黄酮.
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| 关 键 词： | 山蜡梅叶 总黄酮 大孔吸附树脂 |
| 收稿时间： | 2008/10/27 0:00:00 |
Purification of total flavones from leaves of Chimonanthus nitens by macroporous adsorption resins |
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| ZHANG Ya-mei,ZHANG Xiao-juan,JIAN Hui,FENG Yu-lin,WANG Yue-sheng and YANG Shi-lin.Purification of total flavones from leaves of Chimonanthus nitens by macroporous adsorption resins[J].Chinese Traditional and Herbal Drugs,2009,40(8):1226-1229. |
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| Authors: | ZHANG Ya-mei ZHANG Xiao-juan JIAN Hui FENG Yu-lin WANG Yue-sheng and YANG Shi-lin |
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| Institution: | National Pharmaceutical Engineering Center for Solid Preparation in Chinese Materia Medica,Nanchang 330006,China;National Pharmaceutical Engineering Center for Solid Preparation in Chinese Materia Medica,Nanchang 330006,China;National Pharmaceutical Engineering Center for Solid Preparation in Chinese Materia Medica,Nanchang 330006,China;Jiangxi University of Traditional Chinese Medicine,Nangchang 330006, China;National Pharmaceutical Engineering Center for Solid Preparation in Chinese Materia Medica,Nanchang 330006,China;Jiangxi University of Traditional Chinese Medicine,Nangchang 330006, China;National Pharmaceutical Engineering Center for Solid Preparation in Chinese Materia Medica,Nanchang 330006,China;Jiangxi University of Traditional Chinese Medicine,Nangchang 330006, China;National Pharmaceutical Engineering Center for Solid Preparation in Chinese Materia Medica,Nanchang 330006,China;Jiangxi University of Traditional Chinese Medicine,Nangchang 330006, China |
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| Abstract: | Objective To investigate the technological parameters of the purification process of total flavones from the leaves of Chimonanthus nitens with macroporous adsorption resins.Methods Taking the static elution ratio and static absorption ratio as indexes,the static absorption-desorption was used and compared in order to find the optimum macroporous resin.The technical process for purification of total flavones with the optimum macroporous resin was screened by yield of total flavones as index.Results The HPD400 macroporous resin among eight types of resins was found with the best separating efficiency,by which the dynamic absorption capacity could reach 1.68 g(crude drug)/g.Static absorption capacity and desorption ratio were 17.77 mg/g and 92.24%,respectively.After eluted with 4 BV of distilled water and 4 BV of 30% ethanol,eluant was changed with 6 BV of 70% ethanol,the yield of total flavones was 31.4% and product purity was 88.4%.Conclusion With definite technical process,the HPD400 macroporous adsorption resin could be used to purify the extract in the leaves of C.nitens. |
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| Keywords: | the leaves of Chimonanthus nitens Oliv total flavones macroporous adsorption resin |
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