| 柴胡栽培种的RAPD和AFLP遗传关系研究 |
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| 引用本文： | 赵艮贵,南晓洁,郝媛媛,刘小刚,秦雪梅. 柴胡栽培种的RAPD和AFLP遗传关系研究[J]. 中草药, 2010, 41(1): 113-117 |
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| 作者姓名： | 赵艮贵 南晓洁 郝媛媛 刘小刚 秦雪梅 |
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| 作者单位： | 1. 山西大学化学生物学与分子工程教育部重点实验室,山西太原,030006
2. 山西师范大学生命科学学院,山西临汾,041004 |
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| 基金项目： | 国家自然科学基金资助项目(30570174) |
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| 摘 要： | 目的应用不同的分子标记进行柴胡药材干根遗传多样性分析,为柴胡栽培种鉴别和药材鉴别奠定基础。方法以柴胡栽培种干根为材料,采用RAPD和AFLP两种分析方法。结果以筛选到的3条随机引物对9个柴胡栽培种进行RAPD分析,共扩增出28条DNA带,平均每条引物组合扩增9.33条带,其中多态性带为6.67条,多态性比率为71.43%;而在AFLP分析中,筛选出4对特异性引物,共获得107条DNA带,平均每对引物扩增26.75条带,其中多态性带为23.25条,多态性比率为86.92%。经统计分析,9个柴胡栽培种RAPD和AFLP分析的Jaccard遗传相似系数分别介于0.61~0.93和0.39~0.86。UPGMA聚类分析,两种标记方法均可将9个柴胡栽培种聚为3大类群,聚类结果相似但不完全相同。结论以柴胡药材干根为材料,RAPD和AFLP两种分子标记均可用于植物种间及种下遗传关系分析,且AFLP条带较多,多样性丰富,更有利于柴胡属植物多样性的分析。
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| 关 键 词： | 柴胡属 RAPD AFLP 聚类分析 遗传多样性 |
| 收稿时间： | 2009-04-11 |
AFLP Analysis on genetic diversity for germplasm resources of Fritillaria thunbergii cultivated in Zhejiang Province |
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| ZHAO Gen-gui,NAN Xiao-jie,HAO Yuan-yuan,LIU Xiaogang and QIN Xue-mei. AFLP Analysis on genetic diversity for germplasm resources of Fritillaria thunbergii cultivated in Zhejiang Province[J]. Chinese Traditional and Herbal Drugs, 2010, 41(1): 113-117 |
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| Authors: | ZHAO Gen-gui NAN Xiao-jie HAO Yuan-yuan LIU Xiaogang QIN Xue-mei |
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| Affiliation: | ZHAO Gen-gui1,NAN Xiao-jie2,HAO Yuan-yuan1,LIU Xiaogang1,QIN Xue-mei1(1.Key Laboratory of Chemical Biology , Molecular Engineering of Ministry of Education,Shanxi University,Taiyuan 030006,China,2.College of Life Science,Shanxi Normal University,Linfen 041004,China) |
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| Abstract: | Objective To analyze the genetic diversity of Radix Bupleuri using two different molecule markers for distinguishing cultivars in Bupleurum L.and Radix Bupleuri.Methods Random amplified polymorphic DNA(RAPD) and amplified fragment length polymorphism(AFLP) molecular markers were used for distinguishing cultivars in Bupleurum L.and Radix Bupleuri.Results It showed that a total of 28 lines were amplified from nine cultivars in Bupleurum L.using three kinds of primers selected in RAPD analysis,and the average ... |
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| Keywords: | Bupleurum L. RAPD AFLP culster analysis genetic diversity |
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