| 石斛属4种植物的AFLP分析 |
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| 引用本文： | 虞泓,和锐,倪念春,张时刚.石斛属4种植物的AFLP分析[J].中草药,2004,35(7):808-810. |
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| 作者姓名： | 虞泓 和锐 倪念春 张时刚 |
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| 作者单位： | 1. 云南英茂生物技术实验室,云南,昆明,650106;云南大学生命科学学院,生态遗传学实验室,云南,昆明,650091
2. 云南英茂生物技术实验室,云南,昆明,650106 |
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| 摘 要： | 目的 研究石斛属植物DNA指纹图谱,初步探讨AFLP分子标记技术在石斛地道性鉴别上的应用。方法采用扩增片段长度多态性(AFLP)技术对石斛属内石斛组4个种和一个外类群种进行基因组DNA多态性分析。结果从64对引物组合中选出5对引物组合构建了5个种的DNA指纹图谱。通过聚类分析,石斛属4种植物聚成一个大类,彼此关系得到了很好的分辨,并获得bootstrap校验的有力支持。结论AFLP技术可用于药用石斛DNA指纹图谱研究。
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| 关 键 词： | 石斛属 AFLP DNA指纹图谱 |
| 文章编号： | 0253-2670(2004)07-0808-03 |
| 收稿时间： | 2003/10/12 0:00:00 |
Fingerprinting analysis of plants of Dendrobium Sw.by AFLP |
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| YU Hong,HE Rui,NI Nian-chun and ZHANG Shi-gang.Fingerprinting analysis of plants of Dendrobium Sw.by AFLP[J].Chinese Traditional and Herbal Drugs,2004,35(7):808-810. |
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| Authors: | YU Hong HE Rui NI Nian-chun and ZHANG Shi-gang |
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| Institution: | YU Hong 1,2,HE Rui 1,NI Nian-chun 1,ZHANG Shi-gang 1 |
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| Abstract: | Object Studies on DNA fingerprinting of four species of Dendrobium Sw. and the application of amplified fragment length polymorphism (AFLP) to the identification of natural herbal. Methods The polymorphisms of four species from Dendrobium Sw. and one outgroup species were detected by AFLP technique. Results The DNA fingerprinting of five species were generated by fine primer combinations screened from 64 primer combinations. Four species of Dendrobium Sw. were clustered into one big group, whose relationships were distinguished very well. The results were strongly supported by bootstrap test. Conclusion AFLP technique has provided us with the method of study on the DNA fingerprinting of medicinal species of Dendrobium Sw. |
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| Keywords: | Dendrobium Sw amplified fragment length polymoyhism (AFLP) DNA fingerprinting |
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