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车前草中熊果酸、齐墩果酸的HPLC测定
引用本文:袁珂,李根林,李俊芝,杨怡.车前草中熊果酸、齐墩果酸的HPLC测定[J].中草药,1999,30(12):901-903.
作者姓名:袁珂  李根林  李俊芝  杨怡
作者单位:河南中医学院,郑州,450003
基金项目:河南省自然科学基金!第9940240号
摘    要:采用HPLC法同时测定车前草中熊果酸、齐墩果酸的含量。选用C18ODS色谱柱,流动相:甲醇-水(88:12),检测波长:220nm,流速;0.6mL/min,柱温:25℃。熊果酸和齐墩果酸分别在0.4042~2.020μg(4=0.9984)和0.410~2。050μg(r=0.9997)范围内呈线性;熊果酸、齐墩果酸的平均回收率分别为98.2%(RSD=1.74%)97.8%(RSD=/2.24

关 键 词:车前草  熊果酸  齐墩果酸  高效液相色谱
收稿时间:1998/11/30 0:00:00
修稿时间:1998-11-30

Determination of Ursolic Acid and Oleanolic Acid in Asiatic Plantain (Plantago asiatica) by HPLC
Yuan Ke,Li Gen-lin,Li Jun-zhi and Yang Yi.Determination of Ursolic Acid and Oleanolic Acid in Asiatic Plantain (Plantago asiatica) by HPLC[J].Chinese Traditional and Herbal Drugs,1999,30(12):901-903.
Authors:Yuan Ke  Li Gen-lin  Li Jun-zhi and Yang Yi
Institution:He'nan College of TCM, Zhengzhou 450003;He'nan College of TCM, Zhengzhou 450003;He'nan College of TCM, Zhengzhou 450003;He'nan College of TCM, Zhengzhou 450003
Abstract:The contents of ursolic acid and oleanolic acid in Plantago asiatica L. was determined by HPLC. The HPLC system consisted of C_18-ODS, mobile phase of methanol-water (96: 4) mixture, with detection at 220 um, flow rate 0. 6 mL/min and column temperature 25 C. The linear ranges of determination of ursolic acid and oleanolic acid were 0. 404-- 2. 020 ug, r=0. 998 4 and 0. 410-2. 050 ug, r= 0. 999 7, respectively. The mean recovery of 98. 2 %, RSD 1. 74 %, for ursolic acid and 97. 8%, RSD 2. 24% for oleanolic acid is obtained. The method is sensitive, rapid and accurate with good reproducibility.
Keywords:Plantago major L  ursolic acid oleanolic acid  
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