基于HPLC-PDA分析丹参-红花药对配伍的化学物质变化规律

目的采用HPLC-PDA技术和"化学模糊识别"策略对丹参-红花药对不同制法、不同配比的活性物质变化规律进行了系统研究。方法通过建立丹参与红花的化学物质库,并经过对照品峰的紫外信息,建立了4类活性物质的紫外信号网络,并通过网络将丹参-红花中的成分进行快速鉴别与归类,进而通过对称配比设计对这些化合物进行定量分析。结果在丹参-红花配伍的水、50%甲醇水和甲醇煎液中,通过"化学模糊识别"策略分别鉴定出47、57和43个成分。3种不同制法中,丹参酮类成分在甲醇提取液中的相对溶出度最高,其他3类成分在50%甲醇水提取液中的相对溶出度最高。相同制法、不同配比中,所测成分总的相对溶出度的优化范围皆为5∶1~5∶2,总的相对溶出度的最高比例皆为5∶2。结论丹参-红花不同配比与不同制法的化学成分的配伍变化规律有所不同,且存在优化配比,为丹参-红花药对临床配伍合理应用提供了参考,为药对现代基础研究提供了思路与方法。

Chemical interaction analysis on compatibility of Danshen-Honghua pair by using HPLC-PDA

Objective A novel and generally applicable approach was established for the interaction analysis on compatibility of Danshen-Honghua(Salviae Miltiorrhizae Radix and Carthami Flos) pair with different preparations and proportions when decocting together by using HPLC-PDA and fuzzy chemical identification strategy with poly-proportion design.Methods A simple project was initially developed for the rapid identification and classification of four types of components in Danshen-Honghua based on the establishment of relevant component data base,recognition of the reference compound peaks,selection of the characteristics of ultraviolet spectrum,and formation of group networks.In this study,the accurate structures of the chemical components did not need to be determined,and only the constituents attributed to different groups were further considered for quantitative analysis.Results A total of 47,57,and 43 constituents from different preparations of Danshen-Honghua were classified into four kinds of chemical groups,and they were quantitatively analyzed furtherly according to semi-symmetric proportion design.The results showed that the preparation of methanol-water could significantly promote the dissolution of most salvianolic acids,chalcones,and flavonoids,while the contents of tanshinones in methanol were higher than others.The relative dissolution rate range of total optimization for the four types of components were from 5:1 to 5:2,and the highest proportion of the total relative dissolution were all 5:2.Conclusion The most reasonable range may appear 5:1-5:2 for water preparation and be consistent with clinical ratio,which provides the reference for clinical application of Danshen-Honghua pair,and for modern research approach of herb pairs.