| 基于HPLC-Q-TOF-MS研究6种乌头生物碱类成分的裂解途径 |
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| 引用本文： | 孙蕾,王少辰,孙明谦,刘建勋.基于HPLC-Q-TOF-MS研究6种乌头生物碱类成分的裂解途径[J].中草药,2016,47(16):2827-2831. |
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| 作者姓名： | 孙蕾 王少辰 孙明谦 刘建勋 |
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| 作者单位： | 北京中医药大学, 北京 100029;中国中医科学院西苑医院, 中药药理北京市重点实验室, 北京 100091;北京中医药大学, 北京 100029;中国中医科学院西苑医院, 中药药理北京市重点实验室, 北京 100091;中国中医科学院西苑医院, 中药药理北京市重点实验室, 北京 100091;中国中医科学院西苑医院, 中药药理北京市重点实验室, 北京 100091 |
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| 基金项目： | 国家重点基础研究发展计划（973）项目（2015CB554405） |
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| 摘 要： | 目的研究6种乌头类生物碱(乌头碱、次乌头碱、中乌头碱、苯甲酰乌头原碱、苯甲酰次乌头原碱、苯甲酰中乌头原碱)的质谱裂解途径。方法采用高效液相色谱串联四级杆飞行时间质谱(HPLC-Q-TOF-MS)对该类化合物进行分析。结果在正离子模式下,乌头类生物碱的主要裂解途径是连续丢失CH3OH与H2O。双酯型生物碱也可以在C-8位置断裂,失去CH3COOH分子形成明显的特征碎片离子,从而实现与单酯型生物碱的区分。结论此质谱裂解途径可为乌头类生物碱的结构鉴定提供依据,采用HPLC-Q-TOF-MS技术可以高效地分析中药中乌头类生物碱类化学成分,有利于化合物的分析和鉴定。
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| 关 键 词： | 乌头碱 次乌头碱 中乌头碱 苯甲酰乌头原碱 苯甲酰次乌头原碱 苯甲酰中乌头原碱 高效液相色谱-四级杆飞行时间质谱 裂解途径 |
| 收稿时间： | 2015/12/24 0:00:00 |
Fragmentation pathways of six aconitine alkaloids using HPLC-Q-TOF-MS technology |
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| SUN Lei,WANG Shao-chen,SUN Ming-qian and LIU Jian-xun.Fragmentation pathways of six aconitine alkaloids using HPLC-Q-TOF-MS technology[J].Chinese Traditional and Herbal Drugs,2016,47(16):2827-2831. |
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| Authors: | SUN Lei WANG Shao-chen SUN Ming-qian and LIU Jian-xun |
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| Institution: | Beijing University of Chinese Medicine, Beijing 100029, China;Beijing Key Laboratory of Pharmacology of Chinese Materia Medica, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China;Beijing University of Chinese Medicine, Beijing 100029, China;Beijing Key Laboratory of Pharmacology of Chinese Materia Medica, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China;Beijing Key Laboratory of Pharmacology of Chinese Materia Medica, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China;Beijing Key Laboratory of Pharmacology of Chinese Materia Medica, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China |
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| Abstract: | Objective To study the fragmentation pathways of six aconitine-type alkaloids (aconitine, hypaconitine, mesaconitine, benzoylaconitine, benzoylhypaconitine, and benzoylmesaconitine) in mass spectra (MS). Methods The samples were analyzed by liquid chromatography-tandem quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF-MS). Results In positive mode, The typical fragmentation pathways of the six aconitine-type alkaloids were mainly continuous loss of CH3OH and H2O. The characteristic fragmentation pathway of diester-diterpene type aconitine was losing an acetic acid molecule from C-8 position; However this fragmentation could not be observed in MS of mono-ester aconitine alkaloids. Conclusion The study on fragmentation pathways could be adopted for the structural identification of the six aconitine-type alkaloids. |
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| Keywords: | aconitine hypaconitine mesaconitine benzoylaconitine benzoylhypaconitine benzoylmesaconitine liquid chromatography- tandem quadrupole time-of-flight mass spectrometry fragmentation pathways |
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