头花蓼有效组分在大鼠粪便和胆汁中的代谢研究

目的考察头花蓼有效组分在大鼠粪便和胆汁中的代谢转化。方法以头花蓼提取物为研究对象,采用UHPLC-ESI-Q-TOF-MS联用技术,结合Metabolite ToolsTM、质量亏损过滤(MDF)等代谢产物分析技术,系统分析头花蓼有效组分在大鼠粪便和胆汁中的代谢产物。结果在大鼠体内共检出4个原型成分和26个代谢产物,其中粪便、胆汁分别有12、19个成分(含交叉成分),代谢产物主要以槲皮素葡萄糖醛酸化、甲基葡萄糖醛酸化、硫酸化代谢产物为主。结论建立的高分辨质谱方法可以分析头花蓼有效组分在大鼠粪便和胆汁中的代谢产物,初步阐明了头花蓼有效组分的体内代谢特征。

Metabolism of effective fractions of Polygonum capitatum in rat feces and bile

Objective To investigate the metabolite profile of effective fractions of Polygonum capitatum in rat feces and bile.Methods In this study,ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLCESI-Q/TOF-MS) was applied to the identification of metabolites of P.capitatum extracts in rat bile and feces after ig administration.Mass defect filter and Metabolite Tools software were also used.Results The four parent components and 26 metabolites were identified in rats metabolic experiments in vivo,including 12 metabolites in feces and 19 metabolites in bile.The major metabolites were glucuronide conjugation,methyl-glucuronide conjugation,and sulfate conjugation of quercetin.Conclusion The studies have established a high-resolution mass spectrometry method to analyze the metabolites of effective fractions of P.capitatum in vivo.The in vivo metabolic characteristics of the components presented in the fraction have been clarified.The work lays a foundation for the clinical application and development of P.capitatum.