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牛膝的rDNAITS序列分析
引用本文:王淑美,梁生旺,周开亚,刘忠权,冯卫生,吴明侠.牛膝的rDNAITS序列分析[J].中草药,2004,35(5):559-562.
作者姓名:王淑美  梁生旺  周开亚  刘忠权  冯卫生  吴明侠
作者单位:1. 河南中医学院,药物分析学科,河南,郑州,450008
2. 南京师范大学遗传资源研究所,江苏,南京,210097
基金项目:“九五”国家科技攻关项目 ( 99-92 9-0 1-0 6)
摘    要:目的 探讨不同产地牛膝的ITS的序列变异,为鉴别牛膝提供DNA分子标记。方法 利用特异性PCR技术对牛膝的rDNA ITS区碱基序列进行测定,报道了牛膝的rDNA ITS区的碱基序列。结果 得到核糖体DNA中的ITS及5.8S rDNA完全序列,18S和26S rDNA部分序列,共约650bp。牛膝与川牛膝及土牛膝碱基序列有明显差异,不同产地、不同栽培品种牛膝碱基序列无差异。结论 此法可用于牛膝种间及真伪品鉴别。

关 键 词:分子鉴定  牛膝  PCR  序列分析
文章编号:0253-2670(2004)05-0559-04
收稿时间:2003/8/11 0:00:00

Ribosomal rDNA ITS sequence analysis of root of Achyranthes bidentata
Wang Shu-mei,Liang Sheng-wang,Zhou Kai-y,Liu Zhong-quan,Feng Wei-sheng and Wu Ming-xia.Ribosomal rDNA ITS sequence analysis of root of Achyranthes bidentata[J].Chinese Traditional and Herbal Drugs,2004,35(5):559-562.
Authors:Wang Shu-mei  Liang Sheng-wang  Zhou Kai-y  Liu Zhong-quan  Feng Wei-sheng and Wu Ming-xia
Institution:WANG Shu-mei1,LIANG Sheng-wang1,ZHOU Kai-ya2,LIU Zhong-quan2,FENG Wei-sheng1,WU Ming-xia1
Abstract:ObjectTo study the correlation between ITS sequence variation of root of Achyranthes bidentata Blume from different habitat to provide their DNA molecular marker for identification of A. bidentata. Methods To determine rDNA ITS base sequence by peculiar PCR technology. Results The completed sequence of ITS and 5.8 S rDNA, and the partial sequence of 18 S rDNA and 26 S rDNA of root of A. bidentata were obtained. They are about 650 bp. There is obvious diversity among the roots of A. bidentata, Cyathula officinalis Kuan, and A. aspera, while there is no difference among the roots of A. bidentata from different habitat and by different cultivacation breeds. Conclusion The method can be used to identify the root of A. bidentata among different species and to differentiate its fakes.
Keywords:molecular identification  the root of Achyranthes bidentata Blume  PCR  sequence analysis
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