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| 不同产地石斛属种质资源的ISSR遗传多样性分析 | |
| 引用本文： | 卢家仕,卜朝阳,吕维莉,苏建睦,黄昌艳,李春牛.不同产地石斛属种质资源的ISSR遗传多样性分析[J].中草药,2013,44(1):96-100. |
| 作者姓名： | 卢家仕 卜朝阳 吕维莉 苏建睦 黄昌艳 李春牛 |
| 作者单位： | 1. 广西农业科学院花卉研究所,广西南宁530007;广西农业科学院广西作物遗传改良生物技术重点开放实验室,广西南宁530007;广西大学农学院,广西南宁 530004 2. 广西农业科学院花卉研究所,广西南宁,530007 3. 广西农业科学院广西作物遗传改良生物技术重点开放实验室,广西南宁,530007 4. 广西大学农学院,广西南宁,530004 |
| 基金项目： | 农业部热带作物种质资源保护项目(12RZZY-39);广西科技攻关项目(桂科攻1222009-4);广西农科院项目(201014,2011YZ11,2012GW03) |
| 摘 要： | 目的 对24份不同产地石斛属样品的遗传多样性和亲缘关系进行分析.方法 采用ISSR分子标记技术和非加权平均距离法(UPGMA)对24份石斛属样品进行遗传多样性和聚类分析.结果 从100条ISSR引物中共筛选出6条多态性稳定、清晰的引物,24份石斛样品共扩增出847个DNA片段,平均每个引物扩增出141个DNA片段,其多态性为100％.结论 24份不同产地石斛样品被划分为6个类群,遗传多样性非常丰富. |
| 关 键 词： | 石斛属 种质资源 ISSR分子标记 遗传多样性 亲缘关系 |
ISSR analysis on genetic diversity of germplasms resources in Dendrobium SW. from different habitats |
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| LU Jia-shi,BU Zhao-yang,LV Wei-li,SU Jian-mu,HUANG Chang-yan,LI Chun-niu.ISSR analysis on genetic diversity of germplasms resources in Dendrobium SW. from different habitats [J].Chinese Traditional and Herbal Drugs,2013,44(1):96-100. | |
| Authors: | LU Jia-shi BU Zhao-yang LV Wei-li SU Jian-mu HUANG Chang-yan LI Chun-niu |
| Institution: | 1.Flower Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China 2.Guangxi Crop Genetic Improvement and Biotechnology Laboratory,Guangxi Academy of Agricultural Sciences,Nanning 530007,China 3.College of Agronomy,Guangxi University,Nanning 530004,China |
| Abstract: | Objective To analyze the genetic diversity and genetic relationship of 24 plant samples in Dendrobium SW. from different habitats. Methods The molecular marker technique of inter-simple sequence repeat (ISSR) and unweighted pair-group method with arithmetic means (UPGMA) were used to study the genetic diversity and cluster analysis of 24 samples. Results Six primers with stable and clear diversity were sieved from 100 ISSR primers. DNA fragments (847) were amplified from 24 samples, and 141 DNA fragments were amplified from each primer in average, with 100% of polymorphic bands. Conclusion The 24 samples in plants of Dendrobium SW. from different habitats are divided into six groups and the genetic diversity is very abundant. |
| Keywords: | Dendrobium SW germplasms resources ISSR molecular marker genetic diversity genetic relationship |
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