桑黄液体发酵生产多糖工艺研究

目的 考察不同培养条件对桑黄菌液体发酵生产多糖的影响.方法 通过正交试验设计,对不同培养基种类、起始pH值、发酵温度和摇床转速进行优化,在此工艺基础上,采用5L发酵罐进行发酵放大试验.结果 采用添加0.3 9/L维生素B1和0.3 g/L维生素B2的PD培养基,起始pH值5,发酵温度28℃,摇床转速180 r/min条件下,桑黄菌摇瓶发酵液中胞内多糖和胞外多糖分别为5.80、2.37 g/L；5 L发酵罐发酵液中桑黄胞内多糖和胞外多糖分别为5.85、2.30 g/L.结论 桑黄液体发酵可获得桑黄多糖,本实验结果为桑黄液体发酵工业化生产提供参考.

Liquid fermentation of Phellinus igniarius for production of polysaccharide

Objective To investigate the effects of different fermentation conditions on liquid fermentation of Phellinus igniarius for production of polysaccharide.Methods The main fermentation parameters,kinds of media,initial pH values,fermentation temperature,and rotation speed,were optimized by using orthogonal test.Ampliation tests of fermentation were done in a 5 L fermenter.Results The optimal fermentation parameters were potato dextrose(PD) medium with 0.3 g/L vitamin B1 and 0.3 g/L vitamin B2,initial pH value 5,temperature 28 ℃,and rotation speed 180 r/min.Under the optimal fermentation conditions,the maximal intracellular polysaccharide(IPS) and exopolysaccharide(EPS) production was 5.80 and 2.37 g/L in shake flasks,while the maximal IPS and EPS production reached 5.85 and 2.30 g/L in 5 L fermenter,respectively.Conclusion This study provides reference for industrialization of P.igniarius liquid fermentation.