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真菌诱导子对长春花愈伤组织中吲哚生物碱积累的影响
引用本文:张向飞,张荣涛,王宁宁,王勇,王淑芳.真菌诱导子对长春花愈伤组织中吲哚生物碱积累的影响[J].中草药,2004,35(2):201-204.
作者姓名:张向飞  张荣涛  王宁宁  王勇  王淑芳
作者单位:南开大学生命科学学院,天津,300071
基金项目:天津市自然科学基金资助项目 ( 0 13 60 92 11)
摘    要:目的 研究分别来源于镰刀菌Fusarium solani和黑曲霉Aspergillum niger的真菌诱导子(fungal elicitors)对长春花愈伤组织中吲哚生物碱积累的影响。方法 用真菌诱导子对长春花愈伤组织进行诱导处理后提取吲哚总碱,并用RP—HPLC法测定其中阿玛碱和长春质碱含量。结果 两种真菌诱导子对吲哚总碱及其中阿玛碱和长春质碱的积累均有较明显的正向调节作用,并且确立了真菌诱导子最佳处理时间。结论 这两种真菌诱导子对长春花愈伤组织中吲哚生物碱的积累有显著影响。

关 键 词:长春花  真菌诱导子  阿玛碱  长春质碱  愈伤组织
文章编号:0253-2670(2004)02-0201-04
收稿时间:2003/5/12 0:00:00

Effects of fungal elicitors on accumulation of indole alkaloids in Catharanthus roseus calli
ZHANG Xiangfei,ZHANG Rongtao,WANG Ningning,WANG Yong and WANG Shufang.Effects of fungal elicitors on accumulation of indole alkaloids in Catharanthus roseus calli[J].Chinese Traditional and Herbal Drugs,2004,35(2):201-204.
Authors:ZHANG Xiangfei  ZHANG Rongtao  WANG Ningning  WANG Yong and WANG Shufang
Institution:College of Life Science, Nankai University, Tianjin 300071, China;College of Life Science, Nankai University, Tianjin 300071, China;College of Life Science, Nankai University, Tianjin 300071, China;College of Life Science, Nankai University, Tianjin 300071, China;College of Life Science, Nankai University, Tianjin 300071, China
Abstract:Object To investigate the effects of fungal elicitors derived from the fungi Fusarium solani and Aspergillum niger on the accumulation of indole alkaloids in Catharanthus roseus calli. Methods The total indole alkaloid was extracted after the calli were treated with fungal elicitors. Then, the determination of ajmalicine and catharanthine was carried out by RP HPLC. Results The two fungal elicitors stimulate the accumulation not only the total indole alkaloid but the ajmalicine and catharanthine. The optimal exposure time of the two fungal elicitors for different kinds of indole alkaloid was investigated. Conclusion The two fungal elicitors have obvious effect on the accumulation of indole alkaloid in C. roseus calli.
Keywords:Catharanthus roseus (L  ) G  Don  fungal elicitor  ajmalicine  catharanthine  callus
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