| 不同薯蓣皂苷元的盾叶薯蓣遗传关系的RAPD分析 |
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| 引用本文： | 薛焱,秦天才,张友德.不同薯蓣皂苷元的盾叶薯蓣遗传关系的RAPD分析[J].中草药,2006,37(1):115-118. |
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| 作者姓名： | 薛焱 秦天才 张友德 |
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| 作者单位： | 华中农业大学生命科学与技术学院,湖北,武汉,430070 |
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| 基金项目： | 国家自然科学基金资助(30170098) |
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| 摘 要： | 目的从DNA水平分析鉴定不同薯蓣皂苷元盾叶薯蓣的遗传关系。方法从40个10 bp随机引物中筛选出12个引物,进行RAPD分析,应用POPGENE 1.31软件对扩增结果进行聚类分析。结果共扩增出73条DNA片段,其中多态性片段64条,占87.67%,盾叶薯蓣类型间具有明显的多态性差异。结论DNA分子多样性差异与薯蓣皂苷元量差异具有相关性,说明盾叶薯蓣的遗传基础可能对薯蓣皂苷元的形成和积累有显著的影响。
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| 关 键 词： | 盾叶薯蓣 遗传关系 RAPD |
| 文章编号： | 0253-2670（2006）01-0115-04 |
| 收稿时间： | 2005-04-25 |
| 修稿时间： | 2005-04-25 |
Allele-specific diagnostic PCR authentication of Dendrobium huoshanense and its allied species of Dendrobium Sw. |
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| XUE Yan,QIN Tian-cai and ZHANG You-de.Allele-specific diagnostic PCR authentication of Dendrobium huoshanense and its allied species of Dendrobium Sw.[J].Chinese Traditional and Herbal Drugs,2006,37(1):115-118. |
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| Authors: | XUE Yan QIN Tian-cai and ZHANG You-de |
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| Institution: | College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China |
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| Abstract: | Objective To study the genetic relationship among Dioscorea zingiberensis with different diosgenin content at DNA level.Methods DNA of all types was amplified by 12 random primers from 40 random primers,the polymorphic bands of RAPD were counted.And the results of types were analysed by POPGENE 1.31(Tool for Population Genetic Analysis 1.31) software.Results A total of 73 bands was obtained,64 of total bands(87.67%) was polymorphic.The results indicated that there were apparent and abundant genetic variations in D.zingiberensis. Conclusion The formation and accumulation of diosgenin could be affected significantly by the genetic basis of D.zingiberensis. |
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| Keywords: | Dioscorea zingiberensis C H Wright genetic relationship RAPD |
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