一测多评法测定板蓝根中6种化学成分的含量

目的建立板蓝根Isatis indigotica中(R,S)-告依春、直铁线莲宁B、胞苷、鸟苷、尿苷、腺苷6种不同类成分的一测多评方法。方法采用HPLC法,以(R,S)-告依春为内参物,建立了(R,S)-告依春与胞苷、鸟苷、尿苷、腺苷和直铁线莲宁B的相对校正因子,并考察相对校正因子的耐用性和重现性。比较外标法和一测多评法对新疆软紫草中6种成分的测定结果。结果各相对校正因子重复性良好,一测多评法测定结果与外标法测定结果无显著差异。结论建立同时测定6种成分的一测多评法控制板蓝根的质量准确、可靠。

Simultaneous quantitative determination of 10 chemical constituents in Radix isatidis by QAMS method

Objective To establish a new method for the quantitative analysis of multi-components by single-marker (QAMS) and simultaneous determine six different components in Isatis indigotica. Methods The (R, S)-goitrin was used as internal marker to calculate the relative correlation factors (RCF) of clemastanin B, cytidine, uridine, guanosine and adenosine by HPLC, and examine the durability and reproducibility of the RCF. The external standard method and QAMS were compared to determine the six components in I. indigotica. Results The repeatability of RCF was good. The results calculated with QAMS were consistent with the results by the external standard method. Conclusion The QAMS method for simultaneously measuring the content of six components is accurate and reliable to evaluate the quality of I. indigotica.