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| HPLC法测定蛇足石杉中石杉碱甲含量 | |
| 引用本文： | 孙远明,余红英,杨跃生,杨金易,张明.HPLC法测定蛇足石杉中石杉碱甲含量[J].中草药,2002,33(12):1078-1080. |
| 作者姓名： | 孙远明 余红英 杨跃生 杨金易 张明 |
| 作者单位： | 1. 华南农业大学食品科学学院,广东,广州,510642 2. 广东工业大学轻工化工学院 3. 本校生命科学学院 |
| 摘 要： | 目的 考察蛇足石杉不同产地、不同部位中石杉碱甲（Hup A）和总碱含量；比较不同提取方法对石杉碱甲含量的影响。方法 采用RP－HPLC法测定Hup A的含量。结果 Hup A在根、茎、叶中的含量煤戏、叶＞根；贵州、广东和安徽产地的Hup A含量分别为0.018%,0.021%,0.020%，提取方法之间差异不显著。结论 Hup A含量在蛇足石杉中的分布为地上部分大于地下部分。不同产地Hup A含量有一定差异。 |
| 关 键 词： | HPLC法 蛇足石杉 石杉碱甲 石杉总碱 含量测定 |
| 文章编号： | 0253-2670(2002)12-1078-03 |
| 收稿时间： | 2002/4/17 0:00:00 |
| 修稿时间： | 2002年4月17日 |
Determination of hupzine A in Huperzia serrata by HPLC |
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| SUN Yuan-ming,YU Hong-ying,YANG Yue-sheng,YANG Jin-yi and ZHANG Ming.Determination of hupzine A in Huperzia serrata by HPLC[J].Chinese Traditional and Herbal Drugs,2002,33(12):1078-1080. | |
| Authors: | SUN Yuan-ming YU Hong-ying YANG Yue-sheng YANG Jin-yi and ZHANG Ming |
| Institution: | College of Food Science, South China Agriculture University, Guangzhou 510642, China;College of Food Science, South China Agriculture University, Guangzhou 510642, China;College of Food Science, South China Agriculture University, Guangzhou 510642, China;College of Food Science, South China Agriculture University, Guangzhou 510642, China;College of Food Science, South China Agriculture University, Guangzhou 510642, China |
| Abstract: | Object To compare the hupzine A (Hup A) in Huperzia serrata (Thunb.) Trev. obtained by different extracting methods and investigate the amount of alkaloids and the content of Hup A from different parts of the plants and from different places. Methods Using HPLC for the determination of Hup A. Results The content of Hup A in the stem and leaf is richer than that in the root. The content of Hup A from Guizhou, Guangdong and Anhui Provinces is 0.018%, 0.021% and 0.020% repectively; The difference of extract method of Hup A is no prominence. Conclusion The content of Hup A in the ground is richer than that of underground, and there are some difference in the content of Hup A obtained from different places. |
| Keywords: | Huperzia serrata (Thunb ) Trev hupzine A (Hup A) total alkloids HPLC |
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