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分子印迹技术定向分离桂枝茯苓胶囊中活性成分去氢土莫酸
引用本文:倪付勇,刘露,宋亚玲,顾睿,赵祎武,黄文哲,王振中,徐筱杰,萧伟.分子印迹技术定向分离桂枝茯苓胶囊中活性成分去氢土莫酸[J].中草药,2015,46(6):853-856.
作者姓名:倪付勇  刘露  宋亚玲  顾睿  赵祎武  黄文哲  王振中  徐筱杰  萧伟
作者单位:江苏康缘药业股份有限公司, 江苏 连云港 222001;中药制药过程新技术国家重点实验室, 江苏 连云港 222001;中药提取精制新技术重点研究室, 江苏 连云港 222001;江苏康缘药业股份有限公司, 江苏 连云港 222001;中药制药过程新技术国家重点实验室, 江苏 连云港 222001;中药提取精制新技术重点研究室, 江苏 连云港 222001;江苏康缘药业股份有限公司, 江苏 连云港 222001;中药制药过程新技术国家重点实验室, 江苏 连云港 222001;中药提取精制新技术重点研究室, 江苏 连云港 222001;江苏康缘药业股份有限公司, 江苏 连云港 222001;中药制药过程新技术国家重点实验室, 江苏 连云港 222001;中药提取精制新技术重点研究室, 江苏 连云港 222001;江苏康缘药业股份有限公司, 江苏 连云港 222001;中药制药过程新技术国家重点实验室, 江苏 连云港 222001;中药提取精制新技术重点研究室, 江苏 连云港 222001;江苏康缘药业股份有限公司, 江苏 连云港 222001;中药制药过程新技术国家重点实验室, 江苏 连云港 222001;中药提取精制新技术重点研究室, 江苏 连云港 222001;江苏康缘药业股份有限公司, 江苏 连云港 222001;中药制药过程新技术国家重点实验室, 江苏 连云港 222001;中药提取精制新技术重点研究室, 江苏 连云港 222001;北京大学化学与分子工程学院, 北京 100871;江苏康缘药业股份有限公司, 江苏 连云港 222001;中药制药过程新技术国家重点实验室, 江苏 连云港 222001;中药提取精制新技术重点研究室, 江苏 连云港 222001
基金项目:科技部重大新药创制:现代中药创新集群与数字制药技术平台(2013ZX09402203)
摘    要:目的建立利用分子印迹技术从桂枝茯苓胶囊提取物中定向分离制备去氢土莫酸的方法。方法以去氢土莫酸为分子模板,采用溶胶-凝胶法制备了去氢土莫酸分子印迹聚合物,并对其吸附性能进行研究。以此聚合物为填料,从桂枝茯苓胶囊提取物中一步分离制备得到去氢土莫酸,根据理化性质和波谱数据鉴定其结构。结果经Scatchard分析,去氢土莫酸分子印迹聚合物最大表观结合位点数(Qmax)为9.10 mg/g。经HPLC检测去氢土莫酸质量分数为90.76%。结论该方法可用于从桂枝茯苓胶囊提取物中靶向分离制备去氢土莫酸,有利于减少提取过程中有机溶剂的使用,操作简单,为其高效分离纯化提供新的方法。

关 键 词:桂枝茯苓胶囊  分子印迹技术  分子印迹聚合物  溶胶-凝胶法  去氢土莫酸
收稿时间:2/4/2015 12:00:00 AM

Directional separation of active ingredients of dehydrotumulosic acid from Guizhi Fuling Capsule by molecular imprinting technique
NI Fu-yong,LIU Lu,SONG Ya-ling,GU Rui,ZHAO Yi-wu,HUANG Wen-zhe,WANG Zhen-zhong,XU Xiao-jie and XIAO Wei.Directional separation of active ingredients of dehydrotumulosic acid from Guizhi Fuling Capsule by molecular imprinting technique[J].Chinese Traditional and Herbal Drugs,2015,46(6):853-856.
Authors:NI Fu-yong  LIU Lu  SONG Ya-ling  GU Rui  ZHAO Yi-wu  HUANG Wen-zhe  WANG Zhen-zhong  XU Xiao-jie and XIAO Wei
Institution:Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China;The Key Laboratory for the New Technique Research of TCM Extraction and Purification, Lianyungang 222001, China;Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China;The Key Laboratory for the New Technique Research of TCM Extraction and Purification, Lianyungang 222001, China;Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China;The Key Laboratory for the New Technique Research of TCM Extraction and Purification, Lianyungang 222001, China;Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China;The Key Laboratory for the New Technique Research of TCM Extraction and Purification, Lianyungang 222001, China;Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China;The Key Laboratory for the New Technique Research of TCM Extraction and Purification, Lianyungang 222001, China;Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China;The Key Laboratory for the New Technique Research of TCM Extraction and Purification, Lianyungang 222001, China;Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China;The Key Laboratory for the New Technique Research of TCM Extraction and Purification, Lianyungang 222001, China;College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China;Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China;The Key Laboratory for the New Technique Research of TCM Extraction and Purification, Lianyungang 222001, China
Abstract:Objective To establish a method for directional separation of dehydrotumulosic acid from the extracts of Guizhi Fuling Capsule with molecular imprinting technique (MIT). Methods Molecular imprinting polymer (MIP) was prepared by sol-gel process with dehydrotumulosic acid as molecular template to study the absorption property. The dehydrotumulosic acid was achieved from Guizhi Fuling Capsule by one-step separation with polymer as filler. The structure of dehydrotumulosic acid was identified on the basis of the spectral data and physicochemical property. Results The maximum binding capacity (Qmax) of MIP was 9.10 mg/g measured by Scatchard equation and the purity of dehydrotumulosic acid was 90.76% by HPLC. Conclusion The established MIT for the directional separation of dehydrotumulosic acid from Guizhi Fuling Capsule is simple and benefit to reducing the solvent use during the separation process, which could offer a novel method for the separation and purification of dehydrotumulosic acid.
Keywords:Guizhi Fuling Capsule  molecular imprinting technique  molecular imprinting polymer  sol-gel process  dehydrotumulosic acid
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