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| 金钱白花蛇可溶性蛋白凝胶电泳图谱的研究 | |
| 引用本文： | 陈振江,陈科力,王曦,吴和珍,姚明全.金钱白花蛇可溶性蛋白凝胶电泳图谱的研究[J].中草药,2000,31(5):374-376. |
| 作者姓名： | 陈振江 陈科力 王曦 吴和珍 姚明全 |
| 作者单位： | 1. 湖北中医学院药学系,武汉,430061 2. 武汉市一医院药剂科 3. 湖北荆州市第一人民医院药学部 |
| 基金项目： | 湖北省教委自然科学科研项目 |
| 摘 要： | 对金钱白花蛇进行几种凝胶电泳研究，根据聚丙烯酰胺胶电泳（ＰＡＧＥ）谱带的位置和数目进行品种鉴别；用改进的十二烷酸钠－聚丙烯酰胺凝胶电泳（ＳＤＳ－ＰＡＧＥ）测定其主要蛋白质成分分子量；用等电聚焦电泳（ＩＦＥ）测定其主要蛋白质成分的等电点（ＰＩ）。清晰的电泳谱带及相应数据为商品蛇真伪鉴别提供了科学依据。 |
| 关 键 词： | 金钱白花蛇 可溶性蛋白 凝胶电泳图谱 IFE |
| 收稿时间： | 1999/7/16 0:00:00 |
Studies on Gel Electrophoresis Atlas of Soluble Protein in Bungarus |
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| Chen Zhenjiang,Chen Keli,Wang Xi,Wu Hezheng and Yao Mingquan.Studies on Gel Electrophoresis Atlas of Soluble Protein in Bungarus[J].Chinese Traditional and Herbal Drugs,2000,31(5):374-376. | |
| Authors: | Chen Zhenjiang Chen Keli Wang Xi Wu Hezheng and Yao Mingquan |
| Institution: | Wuhan 430061 |
| Abstract: | Disc polyacrylamide gel electrophoresis(PAGE)was performed to detect systematically the soluble proteins in Bungarus. Different species were identified according to the position and number of PAGE bonds.Molecular weights of the soluble proteins were measured by a modified sodium dodecyl sulfonate (SDS) procedure. Isoelectric focusing electrophoresis (IFE) was used to determine the isoelectric point of the main protein of Bungarus. Results showed that the IFE band were always quite distict, which can be used as a scientific basis to distinguish the genuine snakes from its confusable or faked varieties. |
| Keywords: | Bungarus polyacrylamide gel electrophoresis (PAGE) sodium dodecyl sulfonate (SDS) isoelectric focusing electrophoresis (IFE) |
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