北葶苈子化学成分研究

目的研究北葶苈子Lepidium apetalum的化学成分。方法采用硅胶,聚酰胺、反相硅胶C18和Sepha-dex LH-20柱色谱对北葶苈子75%乙醇提取物进行了分离纯化。用质谱、一维及二维核磁等波谱学方法确定了化合物结构。结果从北葶苈子中分离得到11个化合物,分别鉴定为槲皮素-3-O-β-D-2-O-(6-O-芥子酰基)-β-D-吡喃葡萄糖基]-吡喃葡萄糖苷(1)、异鼠李素-3-O-β-D-2-O-(6-O-芥子酰基)-β-D-吡喃葡萄糖基]-吡喃葡萄糖苷(2)、槲皮素-7-O-β-D-吡喃葡萄糖苷(3)、槲皮素-3-O-β-D-吡喃葡萄糖苷(4)、异鼠李素-3-O-β-D-吡喃葡萄糖苷(5)、异鼠李素-7-O-β-D-吡喃葡萄糖苷(6)、山柰酚-7-O-β-D-吡喃葡萄糖苷(7)、槲皮素(8)、β-谷甾醇(9)、胡萝卜苷(10)、蔗糖(11)。结论所有化合物均为首次从该种植物中分离得到。其中化合物1为首次从该属植物中分离得到,化合物2为首次从十字花科植物中分离得到。

Isolation and structure identification of chemical constituents from seeds of Lepidium apetalum

Objective To isolate the chemical constituents from the seeds of Lepidium apetalum and identify their structures. Methods The powdered seeds of L. apetalum were extracted with 70% ethanol and purified by polyamide, silica gel, RP-C_(18) and Sephadex LH-20 column chromatography. Chemical structures were identified by MS, ID, and 2D NMR experiments. Results Eleven compounds were isolated from the seeds of L. apetalum, they are quercetin-3-0-2-0-(6-0-E-sinapoyl)-β-D-glucopymosyl3-β-D-glucopyranoside (1) ,isorhamnetin-3-0- 2-O-( 6-O-E-sinapoyl )-β-D-glucopymosyl ]-β-D-glucopyranoside (2), quercetin-7-O-β-D-glucopyranoside (3), quercetin-3-O-β-D-glucopyranoside (4), isorhamnetin-3-O-β-D-glucopyranoside (5), isorhamnetin-7-O-β-D-glucopyranoside (6), kaempferol-7-O-β-D-glucopyranoside (7), quercetin (8), β-sitpsterol (9), daucosterol (10), and sucrose (11). Conclusion All these compounds are isolated from the seeds of L. apetalum firstly. Compounds 1 and 2 are isolated from the plants of Lepidium L. and Cruciferae plants for the first time, respectively.