毛重楼叶绿体基因组序列特征及其系统发育分析

目的获取毛重楼Parismairei叶绿体基因组信息特征并对其系统位置进行研究。方法采用Illumination测序技术对毛重楼叶绿体基因组进行测序,对其进行组装、注释和特征分析,并构建最大似然(maximumlikelihood,ML)系统发育树。结果毛重楼叶绿体基因组大小为163 918 bp,总GC含量为37.05%,大单拷贝区(large single-copy,LSC)、小单拷贝区(smallsingle-copyregion,SSC)分别为84173、13054bp,反向互补重复区(invertedrepeats,IR)大小为33296bp,共编码113个基因,包括79个蛋白质编码基因,30个t RNA基因和4个r RNA基因。在系统进化树中,重楼属Paris L.与藜芦科(Melanthiaceae Batsch ex Borkh.)关系较近,与百合科(Liliaceae Juss.)关系较远。结论 LSC区和SSC区序列变异高于IR区;相较于重楼属,毛重楼与蚤休组的亲缘关系最近,应将毛重楼归属为蚤休组;相较于百合科,毛重楼与藜芦科藜芦属亲缘关系更近,应将蚤休组从百合科中独立,归属为藜芦科。

Complete chloroplast genome of Paris mairei: characterization and phylogeny

Objective To obtain the complete chloroplast genome information and to estimate phylogenetic relationships among Paris mairei. Methods The Illumina platform was used to sequence the complete chloroplast genome of P. mairei. The bioinformatics software were used to assemble, annotate and characterize the complete chloroplast genome of P. mairei. RAxML was used to reconstruct a maximum-likelihood (ML) phylogenetic tree. Results The complete chloroplast genome of P. mairei was 163 819 bp, containing inverted repeated of 33 296 bp each, a large single-copy region of 84 173 bp, and a small single-copy region of 13 054 bp. A total of 113 genes were identified including 79 protein-coding, 30 tRNAs, and 4 rRNAs. Phylogenetic analysis indicated that Paris presented a closer relationship with Melanthiaceae than Liliaceae. Conclusion The IR region variability was significantly inferior to LSC and SSC. Besides, phylogenetic analysis revealed that P. mairei was closely related to Veratrum.