核酸等温扩增技术及其在中药分子鉴定中的应用研究概况

中药品种的准确鉴定是中药质量控制的首要环节,寻找高效、便捷、准确的鉴定方法,是中药鉴定技术的发展趋势。相对于以表型标记为鉴定特征的传统方法,DNA分子遗传标记鉴定技术具有更加准确、可靠的特点,适用于近缘混淆及多来源品种的鉴定,但同时受PCR技术的限制,存在成本高、步骤复杂、时间长的缺点,无法实现快速鉴定。环介导等温扩增是一种新型核酸扩增技术,具有高特异性、高灵敏性、简便、快速、低成本等优点,成为继PCR技术之后的又一新兴技术,可成功实现对中药材的快速分子鉴定。通过对常见的核酸等温扩增技术及其在中药材分子鉴定研究中的应用情况进行综述,为中药材的快速分子鉴定体系研究提供参考。

Isothermal nucleic acid amplification technology and its application in molecular identification of Chinese materia medica

Accurate identification of varieties is the most important part of quality control of Chinese materia medica (CMM).To find an efficient,convenient,and accurate identification method is the development trend of identification technology of CMM.Compared with the traditional identification method based on phenotypic markers,DNA molecular marker technology is more accurate and reliable,suitable for the identification of closely related species and sample with confusion and multiple sources,but unable to realize the rapid identification due to the limits of the PCR technology,such as high cost,complex procedures,and the drawback of long time.Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification technology,with the advantages of high specificity,high sensitivity,simple,rapid,low cost,etc.,become another new technology after PCR to realize the rapid molecular identification of CMM successfully.In this paper,the common isothermal amplification of nucleic acid technology and its application in the study of molecular identification of Chinese herbal medicines were reviewed analysis,to provide a reference for the study of rapid molecular identification system of Chinese herbal medicines.