浙江产香茶菜属植物8个居群的RAPD分析

目的 从DNA分子水平上探讨香茶菜属植物种群变异特点。方法 从70个随机引物中筛选出10个具有较高多态性检测能力的引物。用这10个随机引物对浙江境内3种香茶菜的8个居群进行了RAPD分析，对得出的RAPD结果应用SPSSl0．0进行的聚类分析和应用CANOC03．10进行的除趋势对应分析。结果 共检测出144个位点，其中单态位点11个，占7．64％，多态位点133个，占92．36％。分析结果表明，浙江产香茶菜不同种、同一种的不同居群间存在明显的遗传分化，其中种间的差异要大于种内，大萼香茶菜Isodon macrocalyx居群间的差异要比香茶菜I.amethystoides居群的小。结论 香茶菜形态与化学成分的变异有一定的遗传学基础。

RAPD analysis of eight populations in plants of Isodon (Schrad. ex Benth.) Kudo from Zhejiang Province

Object To study the population variations from plants of Isodon (Schrad. ex Benth.) Kudo at DNA level. Methods Eight plant populations of Isodon (Schrad. ex Benth.) Kudo collected from Zhejing Province were analyzed by RAPD. The results for eight populations from three species of Isodon (Schrad. ex Benth.) Kudo were analyzed by clustering analysis SPSS10.0 and Detrended Correspondence Analysis (DCA) CANOCO 3.10. Ten of the 70 random primers were tested to possess the stronger detecting effect of polymorphous character. Results A total of 144 bands was amplified by the 10 primers, 7.64% of the total bands was 11 monomorphic, 92.36% of the total bands was 133 polymorphic. The results indicated that the genetic variations existed within the populations of the same species or the different species in Zhejiang Province, and the variances within the populations of I. macrocalyx were slightly smaller than those of I. amethystoides. Conclusion There are some genetic backgrounds in the variations of the morphology and the chemical compounds in the species of I. amethystoides.