| 正交设计在何首乌组织培养中的应用 |
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| 引用本文： | 杜勤,王振华,张俊荣. 正交设计在何首乌组织培养中的应用[J]. 中草药, 1999, 30(7): 537-539 |
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| 作者姓名： | 杜勤 王振华 张俊荣 |
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| 作者单位： | 广州中医药大学中药学院,510407 |
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| 基金项目： | 广东省中医药管理局课题!B96—31 |
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| 摘 要： | 用正交设计法考察了４种激素对何首乌嫩茎，叶诱导愈伤组织产生的影响。方差分析结果显示：２，４－Ｄ、ＩＢＡ作用极显著，６－ＢＡ作用显著，ＮＡＡ作用不显著，它们作用大小依次为：２，４－Ｄ〉ＩＢＡ〉６－ＢＡ〉ＮＡＡ，诱导何首乌愈伤组织产生的最佳激素配比为：ＭＳ＋２，４－Ｄ１ｍｇ／Ｌ＋６－ＢＡ１＋ＩＢＡ０．５。
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| 关 键 词： | 正交设计 何首乌 组织培养 愈伤组织 |
| 收稿时间： | 1998-08-10 |
Application of Orthogonal Design in Tissue Culture of Tuber Fleeceflower(Polygonum multiflorum) |
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| Du Qin,Wang Zhenhua. Application of Orthogonal Design in Tissue Culture of Tuber Fleeceflower(Polygonum multiflorum)[J]. Chinese Traditional and Herbal Drugs, 1999, 30(7): 537-539 |
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| Authors: | Du Qin Wang Zhenhua |
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| Affiliation: | Guangzhou University of TCM, Guangzhou 510470;Guangzhou University of TCM, Guangzhou 510470;Guangzhou University of TCM, Guangzhou 510470 |
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| Abstract: | Effects of 2, 4-D, 6-BA, NAA and IBA on calli inducement of Polygonum multiflorum Thunb. were studied by L_9(3~4) orthogonal design. The experimental results showed that the actions of 2,4D and IBA were extremely remarkable, that of 6-BA was remarkable, while NAA was least effective. The order of their efficiencies was 2, 4-D>IBA > 6-BA> NAA. The best medium for calli inducement was MS 2,4-D, 1 mg/L 6-BA, 1 mg/L IBA, 0.5 mg/L. |
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| Keywords: | orthogonal design Polygonum multiflorum Thunb. tissue culture callus |
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