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不同种质金线莲氨基酸和矿物质元素量的比较
引用本文:许梦洁,叶申怡,吴梅,邵清松,谭欣,李明焱.不同种质金线莲氨基酸和矿物质元素量的比较[J].中草药,2017,48(2):368-372.
作者姓名:许梦洁  叶申怡  吴梅  邵清松  谭欣  李明焱
作者单位:浙江农林大学中药学院, 浙江 杭州 311300;浙江农林大学中药学院, 浙江 杭州 311300;金华市农业科学研究院, 浙江 金华 321017;浙江农林大学中药学院, 浙江 杭州 311300;浙江农林大学 亚热带森林培育国家重点实验室培育基地, 浙江 杭州 311300;浙江农林大学中药学院, 浙江 杭州 311300;浙江省珍稀植物药工程技术研究中心, 浙江 武义 321200
基金项目:国家自然科学基金项目(81303167,81673531);浙江省农业新品种选育重大科技专项(2016C02058);浙江省大学生科技创新活动(新苗人才)计划基金项目
摘    要:目的比较不同种质金线莲中氨基酸和矿物质元素的差异。方法采用全自动氨基酸分析仪和原子吸收光谱仪测定11个不同种质金线莲中氨基酸和矿物质元素的量。结果不同种质金线莲必需氨基酸为2.81%~4.47%,总氨基酸量为11.38%~17.06%,其中天门冬氨酸、谷氨酸和精氨酸的量明显高于其他氨基酸组分。矿物质元素中钾元素的量最高,其顺序为KCaMgFeMnZnCrCuPbCd。主成分分析表明丙氨酸、丝氨酸、谷氨酸、天门冬氨酸、苏氨酸、异亮氨酸、丙氨酸、苯丙氨酸、亮氨酸为金线莲的特征性氨基酸,Fe、Zn、Mn、Ca、Cr、Mg为金线莲的特征性元素。结论金线莲中氨基酸和矿物质元素量存在地域性差异。

关 键 词:金线莲  氨基酸  矿物质元素  主成分分析  聚类分析
收稿时间:2016/6/19 0:00:00

Comparison on contents of mineral elements and amino acids in different germplasms of Anoectochilus roxburghii
XU Meng-jie,YE Shen-yi,WU Mei,SHAO Qing-song,TAN Xin and LI Ming-yan.Comparison on contents of mineral elements and amino acids in different germplasms of Anoectochilus roxburghii[J].Chinese Traditional and Herbal Drugs,2017,48(2):368-372.
Authors:XU Meng-jie  YE Shen-yi  WU Mei  SHAO Qing-song  TAN Xin and LI Ming-yan
Institution:Department of Chinese Materia Medica, Zhejiang A & F University, Hangzhou 311300, China;Department of Chinese Materia Medica, Zhejiang A & F University, Hangzhou 311300, China;Jinhua Academy of Agricultural Sciences, Jinhua 321017, China;Department of Chinese Materia Medica, Zhejiang A & F University, Hangzhou 311300, China;Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Hangzhou 311300, China;Department of Chinese Materia Medica, Zhejiang A & F University, Hangzhou 311300, China;Rare Herbal Medicine and Engineering Institute of Zhejiang Province, Wuyi 321200, China
Abstract:Objective To compare the contents of mineral elements and amino acids in different germplasms of Anoectochilus roxburghii. Methods The contents of amino acids and mineral elements in 11 different germplasms of A. roxburghii were determined by automatic amino acid analyzer and atomic absorption spectrometer. Results The range of essential amino acids in the different species of A. roxburghii was 2.81%-4.47%, and the range of total amino acids was 11.38%-17.06%. Aspartic acid, glutamic acid, and lysine contents were significantly higher than those of other amino acids. The content of potassium was the highest, and the order was:K > Ca > Mg > Fe > Mn > Zn > Cr > Cu > Pb > Cd. The principal components analysis showed that alanine, serine, glutamic acid, aspartic acid, threonine, isoleucine, alanine, phenylalanine, and leucine were characteristic amino acids in A. roxburghii. Fe, Zn, Mn, Ca, Cr, and Mg were characteristic elements of A. roxburghii. Conclusion There are regional differences of A. roxburghii between amino acids and the content of mineral elements.
Keywords:Anoectochilus roxburghii(Wall  ) Lindl    amino acids  mineral elements  principal components analysis  cluster analysis
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