高效毛细管电泳法测定苦参碱和氧化苦参碱

建立了测定苦参碱和氧化苦参碱含量的高效毛细管电泳法。２０ｍｍｏｌ／Ｌ的磷酸盐缓冲液为电泳电解质，电压为３０ｋＶ（运动电充为１００－１１０μＡ），检测波长为２１４ｎｍ，内标氨茶碱。苦参碱０．１２６－１．２６ｍｇ／ｍＬ，氧化苦参碱０．１０－１．０ｍｇ／ｍＬ其浓度与峰面积之比呈良好的线性关系，ｒ均为０．９９９８，日内，日间变异系数均小于５．７％，常用２２种药物除地塞米松外其余的对其无干扰。

Determination of Matrine and Oxymatrine by HPCE

A method has been developed for the determination of matrine and oxymatrine by high performance capillary electrophoresis (HPCE).Aminophy-lline was used as internal standard.Complete separation was achieved with 20 mmol/L running phosphate buffer (pH=8.0) at a constant voltage of 30 kV (current of 100~ 110 PA) and detected at a wavelength of 214 nm.Good linearity was shown between the concentration and peak area in the concentration range of 0.126-1.26 mg/mL for matrine and 0.10~ 1.0 mg/mL for oxymatrine,r=0.9998.The relative standard deviation (RSD) for within-day and between-day was less than 5.7 %.22 different drugs in common use except dexamethasone showed no interference.Two samples of extract of Sophora flavescens Ait.gave results of (45.19±0.058) % and (62.81±0.123) % for matrine and (8.71±0.03) % and (6.81± 0.086)% for oxymatrine on a trial determination.It seemed that the HPCE method is faster,more convenient and economical as compared with HPLC.