| 新疆药用桑树9个栽培群体的RAPD分析 |
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| 引用本文： | 傅大煦,张辉,陈纹,苏雪,孙坤,陈家宽.新疆药用桑树9个栽培群体的RAPD分析[J].中草药,2004,35(9):1053-1056. |
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| 作者姓名： | 傅大煦 张辉 陈纹 苏雪 孙坤 陈家宽 |
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| 作者单位： | 1. 复旦大学生物多样性科学研究所,上海,200433
2. 西北师范大学植物研究所,甘肃,兰州,730070 |
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| 摘 要： | 目的 从DNA分子水平上分析新疆药用桑树资源9个栽培群体的遗传关系。方法 从100个随机引物中筛选出10个引物，用这10个引物做RAPD扩增，应用NTSYS软件对扩增结果进行聚类分析。结果 共扩增出108个位点。其中多态性位点91个，多态比例为84．26％，各群体间存在较明显的遗传分化。另外，获得药桑的8个特异位点。结论 RAPD分析结果与新疆药用桑树资源植物的遗传关系的传统划分是基本一致的。
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| 关 键 词： | 桑树 栽培群体 RAPD 遗传多样性 |
| 文章编号： | 0253-2670(2004)09-1053-04 |
| 收稿时间： | 2004/2/12 0:00:00 |
RAPD analysis of n ine populations of medicinalmulberry cultivated in Xinjiang |
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| FU Daxu,ZHANG Hui,CHEN Wen,SU Xue,SUN Kun and CHEN Jiakuan.RAPD analysis of n ine populations of medicinalmulberry cultivated in Xinjiang[J].Chinese Traditional and Herbal Drugs,2004,35(9):1053-1056. |
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| Authors: | FU Daxu ZHANG Hui CHEN Wen SU Xue SUN Kun and CHEN Jiakuan |
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| Institution: | Institute of Biodiversity Sciences, Fudan University, Shanghai 200433, China;Institute of Botany, Northwest Normal University, Lanzhou 730070, China;Institute of Botany, Northwest Normal University, Lanzhou 730070, China;Institute of Botany, Northwest Normal University, Lanzhou 730070, China;Institute of Botany, Northwest Normal University, Lanzhou 730070, China;Institute of Biodiversity Sciences, Fudan University, Shanghai 200433, China |
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| Abstract: | Object To study the inherited relationships among nine medicinal mulberry populations cultivated in different regions of Xinjiang at DNA level Methods DNA of all populations was amplified by ten random primers from 100 ramdom primers, the polymorphic bands of RAPD were counted And the results of populations were analyzed by clustering analysis NTSYS PC software Results A total of 108 bands was obtained, 91 of total bands (84 26%) was polymorphic The results indicated that there were apparent and abundant genetic variations in Morus L of Xinjiang Besides, eight specific bands of M nigra were obtained Conclusion The genetic relationship among medicinal resources plants of mulberry in Xinjiang indicats by the RAPD analysis to be consistent with their traditional classifications in the main |
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| Keywords: | mulberry cultivated population RAPD genetic diversity |
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