苏木的化学成分研究

目的研究苏木Caesalpinia sappan的化学成分。方法采用硅胶、葡聚糖凝胶Sephadex LH-20柱色谱、半制备液相色谱等方法分离纯化,通过理化性质、波谱数据及与对照品共薄层色谱鉴定化合物结构。结果从苏木心材95%乙醇提取物的石油醚萃取部位分离并鉴定了11个化合物,分别为1-羟基-7-甲氧基呫吨酮(1)、2-羟基呫吨酮(2)、1,5-二羟基呫吨酮(3)、1,7-二羟基呫吨酮(4)、大黄酚(5)、木栓酮(6)、熊果酸(7)、β-谷甾醇棕榈酸酯(8)、麦角甾烷-4,6,8(14),22(23)-四烯-3-酮(9)、豆甾醇(10)、β-谷甾醇(11)。结论除化合物10、11外,其余均为首次从该植物中分离得到,其中化合物1～5为首次从云实属植物中分离得到,同时也是该属植物中首次分得的呫吨酮和蒽醌类化合物。

Study on chemical constituents from heartwood of Caesalpinia sappan

Objective To study the chemical constituents from the heartwood of Caesalpinia sappan.Methods The chemical constituents were isolated and purified by column chromatography on silica gel,Sephadex LH-20,and semi-preparative HPLC.The structures of compounds were identified by physicochemical properties,spectral analysis,and co-TLC with reference substances.Results Eleven compounds were isolated from the petroleum ether fraction of 95% ethanol extract and were identified as 1-hydroxy-7-methoxy xanthone(1),2-hydroxyxanthone(2),1,5-dihydroxyxanthone(3),1,7-dihydroxyxanthone(4),chrysophanol(5),friedelin(6),ursolic acid(7),β-sitosterol palmitate(8),ergosta-4,6,8(14),22(23)-tetraen-3-one(9),stigmasterol(10),and β-sitosterol(11).Conclusion Except compounds 10 and 11,others are isolated from this plant for the first time.Compounds 1—5 are firstly isolated from the genus Caesalpinia L.and are also compounds with xanthone and anthraquinone skeletons firstly isolated from the plants of Caesalpinia L.