老鸦瓣芽茎组织培养初步研究

目的 建立老鸦瓣芽茎愈伤组织诱导及丛生芽增殖体系。方法 以老鸦瓣冷藏芯芽产生的芽茎为外植体，MS为基本培养基，考察不同质量浓度6-BA、NAA对愈伤诱导、分化及丛生芽增殖的影响。结果 芽茎诱导愈伤的最佳培养基为MS＋6-BA 0.5 mg/L＋NAA 2.0 mg/L，愈伤诱导率78.54%，诱导出的愈伤组织在原培养基上继代培养增殖后即可进行芽分化；愈伤分化不定芽最佳培养基为MS＋6-BA 2.0 mg/L＋NAA 0.2 mg/L，芽诱导率为66.21%；丛生芽增殖培养基为MS＋6-BA 0.5 mg/L＋NAA 0.2 mg/L，增殖系数2.48。结论 筛选出芽茎诱导愈伤、分化不定芽及丛生芽增殖的培养基，初步建立了老鸦瓣芽茎组织培养体系。

Bud stem tissue culture of Tulipa edulis

Objective To establish the tissue culture system of callus induction and cluster shoot proliferation with bud stems of Tulipa edulis. Methods Bud stems were isolated from cooled T. edulis bulbs as explants. The calli were inducted on MS media with different concentration of 6-BA and NAA, and the cultural conditions of shoot differentiation and multiplication were optimized. Results The optimal medium for callus induction was MS + 6-BA 0.5 mg/L + NAA 2.0 mg/L with a callus inducation rate of 78.54%. After subcultured in original medium, the callus was turned into differentiation medium. The optimal medium for callus differentiation was MS + 6-BA 2.0 mg/L + NAA 0.2 mg/L with a shoot differentiation rate of 66.21%. The optimal medium for the shoot multiplication was MS + 6-BA 0.5 mg/L + NAA 0.2 mg/L, and the proliferation coefficient was 2.48. Conclusion The media for callus induction, adventitious bud differentiation, and cluster shoot proliferation are optimized. The optimal medium for the culture of T. edulis bud stems is preliminarily established.