油茶皂苷对LO2和HK-2细胞的毒性作用研究

目的:观察油茶皂苷(SQS)对人正常肝细胞(LO2)和肾小管上皮细胞(HK-2)的细胞毒性作用,并初步探讨其毒性作用机制。方法:体外培养LO2和HK-2细胞,采用MTT法评价SQS对LO2和HK-2细胞的增殖抑制作用,观察不同浓度药物对乳酸脱氢酶(LDH)释放率的影响,并检测细胞裂解上清液超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量。结果:与阴性对照组比较,各浓度的SQS均可明显抑制LO2和HK-2细胞增殖(P<0.01),且呈浓度依赖性。在50.0~200.0 mg/L范围内,SQS能明显提高LO2和HK-2细胞培养上清液中LDH活力(P<0.05或P<0.01),显著降低SOD的活力和增加MDA的含量(P<0.01)。结论:SQS对LO2和HK-2细胞有一定的细胞毒性作用,其作用机制可能是通过增加对细胞膜通透性的影响及氧化损伤而实现。

Study on cytotoxicity of LO2 and HK-2 cells by sasanquasaponin

Objective： To observe the cytotoxic effect of sasanquasaponin （SQS） on normal liver cells（L02） and the renal tubular epithelial cells （HK-2）,and make a preliminary study on the mechanism of its cytotoxicity. Methods： LO2 and HK-2 cells were cultured in vitro. MTY assay was used to evaluate the anti-proliferative effect of SQS on LO2 and HK-2 cells. The effect of drugs with different concentration on release rate of lactate dehydrogenase（LDH） was observed. The activity of superoxide dismutase（SOD） in superuatant of cell lysis and the content of malondialdehyde （MDA） were detected as well. Result： Compared with the negative control group, different concentration of SQS could obviously inhibit proliferation of LO2 and HK-2 cells in a concentrationdependent manner（P〈0.01 ）. SQS could markedly increase the levels of LDH（P〈0.05 or P〈0.01 ） in the supernatant of cell culture fluid,significantly decrease the activity of SOD and increase the content of MDA （P〈0.01） 50.0～200.0 mg/L. Conclusion： SQS poses certain cytotoxic function on L02 and HK-2 cells. Its possible mechanism is that SQS can increase the permeability and oxidative damage of LO2 and HK-2 cells.