彝药“麻补”止血活性物质基础及机理研究

目的：阐明彝药“麻补”（狭叶重楼）的止血活性物质基础及机理。方法：采用植物化学技术从“麻补”药材中分离鉴定出一种C27甾体皂苷类化合物——重楼皂苷H（PSH）。测定了PSH 对正常小鼠的断尾出血时间（BT）、凝血酶原时间（PT）、活化的部分凝血活酶时间（APTT）、血浆纤维蛋白原（FIB）等凝血功能指标的影响。结果：PSH 可显著缩短BT，显示出明显的止血活性；PSH 对缩短正常小鼠PT、APTT 作用不明显，但可显著提高FIB。结论：重楼皂苷H 对内源凝血通路及外源凝血通路无明显促进作用，PSH 升高FIB 为其发挥止血活性的重要途径。

Research on Material Basis And Mechanism for Hemostatic Activity of Yi Medicine "Ma-Bu"

This study was aimed to carry out pharmacological research on the hemostatic activity and mechanism of Yi medicine "Ma-Bu" (Paris polyphylla Smith var stenophylla Franch.). One kind of C27 steroidal saponin from P.polyphylla Smith var stenophylla Franch. was isolated and identified as Paris saponin H (PSH). The effect of PSH on the index of bleeding time (BT), prothrombin time (PT), activated partial thromboplastin time (APTT) and fibrinogen (FIB) of mice were measured. The results showed that PSH have significant hemostatic activity by shortening BT. The effect of PSH on shortening PT and APTT of mouse was not significant. However, the FIB was enhanced significantly after treatment with PSH. It was concluded that PSH had no significant promoting effect on the extrinsic coagulation pathway (ECP) or the intrinsic coagulation pathway (ICP). The enhancement of FIB may be a pathway for the effect of hemostatic activity by PSH.