铁皮石斛提取物促进肌肉损伤修复作用的机理研究

目的：利用C2C12成肌细胞和大鼠骨骼肌体外实验体系，研究铁皮石斛提取物对运动性损伤修复作用的机理。方法：用不同浓度铁皮石斛水提物处理C2C12细胞，进行划痕实验以及提取细胞蛋白检测其 EGFR的表达水平。通过手术取大鼠滑车上肌，用石斛水提物对其进行体外培养并检测其EGFR表达水平情 况。结果：C2C12细胞划痕实验结果显示，石斛提取物处理2 h后对C2C12迁移增殖有一定促进作用，其作用效 果与浓度相关。石斛提取物处理后的C2C12细胞EGFR表达有一定的增加趋势。体外培养肌肉组织结果表 明，铁皮石斛水提物能显著提高肌肉组织中EGFR的表达水平。结论：实验结果表明铁皮石斛水提物通过正向 调控C2C12细胞和骨骼肌组织的EGFR表达水平，起到促进骨骼肌细胞增殖的作用，可以对骨骼肌运动性损伤 起到一定的修复作用。

Study on Recovery of Skeletal Muscle Cell Injured by Dendrobium Candidum Extract

Objective: To study the recovery effects of skeletal muscle cell injured and its molecular mechanism of dendrobium candidum extract on C2C12 myotubes and isolated rat skeletal muscle. Method: In this study, we adopted different dendrobium candidum extract on C2C12 cells cultured and scratch test, the expression level of EGFR(epidermal growth factor receptor) was examined. Isolated rat epitrochlearis muscle was incubated in a buffer containing dendrobium candidum extract and the EGFR activation and related events were examined. Result: The results of scratch test showed that dendrobium candidum extract(2h) promoted the migration and proliferation of C2C12 cells, and its effect was related in a dose-dependent manner. And the EGFR expression level of C2C12 cells treated with dendrobium candidum extract was increased. In response to dendrobium candidum extract treatment, expression level of EGFR increased in rat epitrochlearis muscles. Conclusion: Dendrobium candidum extract stimulates C2C12 cells and skeletal muscle EGFR activity acutely, promoted the migration and proliferation of C2C12 cells, and been shown to have recovery effects of skeletal muscle cell injured.