| 中国黄芪属药用植物DNA条形码（ITS2）鉴定 |
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| 引用本文： | 高 婷,姚 辉,马新业,朱英杰,宋经元.中国黄芪属药用植物DNA条形码（ITS2）鉴定[J].世界科学技术-中医药现代化,2010,12(2):222-227. |
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| 作者姓名： | 高 婷 姚 辉 马新业 朱英杰 宋经元 |
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| 作者单位： | 中国医学科学院北京协和医学院药用植物研究所 北京 100193 |
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| 基金项目： | 科技部卫生行业科研专项（200802043）：药用植物DNA barcoding(条形码)鉴定研究，负责人：陈士林。 |
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| 摘 要： | 中国黄芪属植物种类繁多，具有重要的药用及生物学价值。本文采用了植物DNA条形码候选序列之一的ITS2片段来探讨在黄芪属药用植物中的鉴定能力。结果显示，ITS2基因区通用性强，序列在黄芪属物种间的差异较大，具有明显的Barcoding Gap，能够正确鉴定41个黄芪属植物物种，仅6个物种不能鉴定。此外，ITS2的二级结构也具有一定的系统学及分类学意义。本研究表明ITS2能够作为黄芪属药用植物鉴定的DNA条形码序列，具有重要的应用价值。
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| 关 键 词： | ITS2序列 黄芪属 鉴定 DNA条形码 |
| 收稿时间： | 4/2/2010 12:00:00 AM |
| 修稿时间： | 2010/4/19 0:00:00 |
Identification of Astragalus Plants in China Using the Region ITS2 |
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| Gao Ting,Yao Hui,Ma Xinye,Zhu Yingjie and Song Jingyuan.Identification of Astragalus Plants in China Using the Region ITS2[J].World Science and Technology-Modernization of Traditional Chinese Medicine,2010,12(2):222-227. |
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| Authors: | Gao Ting Yao Hui Ma Xinye Zhu Yingjie and Song Jingyuan |
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| Institution: | Institute of Medicinal Plant Development, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100193, China |
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| Abstract: | Astragalus is a large genus in China, containing various medicinal plant species. In this study, we tested the applicability of a DNA barcode ITS2 for identifying medicinal plant species within the Astragalus genus. The result showed that ITS2 has great universality, proper intra- /inter-specific divergence, obvious Barcoding Gap, and strong species discrimination power. Based on the BLAST1 method, 41 of 47 Astragalus species have been identified by ITS2. In addition, ITS2 secondary structure is of significance for the systematic and taxonomic study. Therefore, ITS2 may be a useful barcode for Astragalus species, with great application value. |
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| Keywords: | ITS2 Astragalus Identificaion DNA barcoding |
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