北五味子仁提取物对高胆固醇血症小鼠脂质代谢的影响

目的：研究北五味子仁石油醚提取物（A1）、乙醇提取后石油醚提取物（A2）、乙醇提取物（A3）和石油醚提取后乙醇提取物（A4）对高胆固醇血症小鼠脂质代谢的影响。方法：将雄性ICR 小鼠分为11组（n=10），分别为正常饲料组、高脂饲料组、高脂饲料+1%或4%A1、A2、A3、A4 组和高脂饲料+0.1%非诺贝特（阳性药）组。饲养10 天后按照试剂盒说明书测定血清和肝脏脂质水平。结果：高脂饲料组小鼠血胆固醇（TC）、低密度脂蛋白（LDL）水平，以及胆囊和粪便中TC 含量显著升高。非诺贝特可显著降低高胆固醇血症小鼠血/肝脏脂质水平及胆囊中TC 含量，但增加粪便中TC 的排出量，并显著升高血清丙氨酸转移酶（ALT）活性。A1 具有升高高胆固醇血症小鼠的血脂和肝重量的作用。A2 和A3 降低高脂饲料组小鼠血TG水平，A4 降低血清中TC 和LDL 水平。北五味子仁4 种提取物均可显著降低胆囊TC 水平，A2、A3 和A4增加粪便TC 含量。结论：北五味子仁提取物可显著影响高胆固醇血症小鼠的脂质代谢，促进TC 排泄。

Influent of Fructus Schisandrae Seed Extracts on Lipid Metabolism in Hypercholesterolemic Mice

This study was aimed to investigate effects of Fructus Schisandrae seed extracts, including petroleum ether extract (A1), petroleum ether extract after ethanolic extraction (A2), ethanolic extract (A3) and ethanolic extract after petroleum ether extraction (A4), on lipid metabolism in hypercholesterolemic mice. Male ICR mice were divided into 11 groups with 10 mice in each group. Mice were fed with normal diet and high-cholesterol/bile salt diet (HCBD) supplemented without or with 1% and 4% A1, A2, A3 and A4 or 0.1% fenofibrate, respectively, for 10 days. Serum and hepatic lipids were determined according to the manufacturer's instruction. The results showed that the serum level of total cholesterol (TC), low-density lipoprotein (LDL), and TC content in the gallbladder and feces were obvious increased in mice fed with HCBD. Fenofibrate treatment can significantly reduce serum/hepatic lipids, TC content in the gallbladder, but increase TC content in the feces in the hypercholesterolemic mice. It also obviously increased the serum alanine aminotransferase (ALT) activity. A1 had the effect of increasing blood lipid level and liver weight of hypercholesterolemic mice. A2 and A3 can reduce TC level of mice fed with HCBD. A4 reduced serum TC and LDL level. Four kinds of Fructus Schisandrae seed extracts can significantly reduce the TC level in the gallbladder. A2 and A4 increased TC content in the feces. It was concluded that the Fructus Schisandrae seed extracts can significantly influence lipid metabolism in hypercholesterolemic mice and promote TC excretion.