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ITS2序列鉴定筋骨草及其近缘种
引用本文:韩建萍,石林春,陈士林,钱俊,罗焜,陈科力.ITS2序列鉴定筋骨草及其近缘种[J].世界科学技术-中医药现代化,2008,10(6):86-89.
作者姓名:韩建萍  石林春  陈士林  钱俊  罗焜  陈科力
作者单位:中国医学科学院北京协和医学院药用植物研究所,北京100193;中国医学科学院北京协和医学院药用植物研究所,北京100193;湖北中医学院,武汉430065;湖北中医学院,武汉430065
基金项目:中央级公益性科研院所基本科研业务专项(YZ01-1):川贝母EST文库构建和hmgr基因克隆研究,负责人:韩建萍
摘    要:本研究对筋骨草ITS2区段测序,探讨该片段用于中药筋骨草、白苞筋骨草、白毛夏枯草和夏枯草种间分子鉴别和系统学研究中的意义,我们对核基因ITS2区段进行PCR扩增并测序,获得了该区间的完整序列,所得序列用软件MEGA4.0进行相关分析。测得筋骨草、白苞筋骨草、夏枯草和白毛夏枯草的ITS2片段长度分别为312bp,304bp,303bp,304bp。四个物种ITS2片段序列的遗传距离(Kimura2)为0.010~0.175,各个筋骨草种内的不同产地该序列无差异。用MP法根据ITS2序列的遗传距离建立系统发生树,聚类结果和形态分类相符。根据ITS2序列特征,能有效区分筋骨草和其他易混中药材。ITS2具有做为鉴定的条形码序列的潜力。应用该序列可以有效鉴定筋骨草及其近缘种。

关 键 词:ITS2序列    筋骨草    序列分析    鉴定  
收稿时间:2008/3/24 0:00:00
修稿时间:4/1/2008 12:00:00 AM

Authentication of Ajuga ciliate and Its Close Species Using ITS2 Sequence
Han Jianping,Shi Linchun,Chen Shilin,Qian Jun,Luo Kun and Chen Keli.Authentication of Ajuga ciliate and Its Close Species Using ITS2 Sequence[J].World Science and Technology-Modernization of Traditional Chinese Medicine,2008,10(6):86-89.
Authors:Han Jianping  Shi Linchun  Chen Shilin  Qian Jun  Luo Kun and Chen Keli
Institution:Institute of Medicinal Plant Development; Peking Union Medical College & Chinese Academy of Medical Sciences; Beijing; P.R.China;Institute of Medicinal Plant Development; Peking Union Medical College & Chinese Academy of Medical Sciences; Beijing; P.R.China;Hubei University of Chinese Medicine; Wuhan; 430065; P.R.China;Hubei University of Chinese Medicine; Wuhan; 430065; P.R.China
Abstract:This is an article to discuss the value of the fragments used in molecular identity and systematics of four species(Ajuga ciliata Ajuga lupulina,Prunella vulgaris,Ajuga decumbens).The ITS2 fragments of the three species were amplified and sequenced,and were analyzed using software MEGA4.0.The length of Ajuga ciliata Ajuga lupulina,Prunella vulgaris,Ajuga decumbens fragments was 312 bp,304 bp,303bp and 304bp,respectively.The interspecific genetic distance(Kimura -distance) of the three species was 0.010 -0.1...
Keywords:ITS2  Ajuga ciliate  sequence analysis  authentication
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