| 甘遂对正常小鼠肝脏相关炎性因子表达的影响 |
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| 引用本文： | 卢希,雷帆,邢东明,王玉刚,肖新月,胡珺,程显隆,柴玉爽,冯天师,杜力军.甘遂对正常小鼠肝脏相关炎性因子表达的影响[J].世界科学技术-中医药现代化,2012,14(3):1636-1641. |
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| 作者姓名： | 卢希 雷帆 邢东明 王玉刚 肖新月 胡珺 程显隆 柴玉爽 冯天师 杜力军 |
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| 作者单位： | 1. 清华大学生命科学学院医学院药物药理研究室/蛋白质科学教育部重点实验室 北京100084
2. 中国食品药品检定研究院 北京 100050 |
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| 基金项目： | 科学技术部国家“十一五”科技支撑项目（2006BAI14B01）：药品安全关键技术研究———常见与重要药品安全标准研究，负责人：肖新月；科学技术部国家“十一五”创新药物重大专项（2009ZX09502）：中药研究关键技术———利用转基因技术构建特定靶点细胞系评价中药致敏性的探索，子课题负责人：雷帆。 |
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| 摘 要： | 目的:观察甘遂醇提取物对正常小鼠肝脏功能及其相关炎性因子表达的影响,以探讨其对肝脏的安全性.方法:分别给小鼠灌胃给药(1.2、0.6、0.3 g·kg-1)2 h、4h和7d,取血清测ALT,取肝脏做组织学观察,并提取RNA,Real time PCR测IL-β、TNFα和Caspase3表达的变化.结果:甘遂醇提取物给药不同时间对正常小鼠肝脏组织形态影响不明显,给药2h大剂量对ALT明显升高,对炎性因子IL-β的mRNA表达明显上调,对凋亡相关因子Caspase3的mRNA表达影响不明显.结论:甘遂对正常小鼠肝脏具有确实的毒性.其作用特点主要与炎性损伤有关,与凋亡关系不明显.
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| 关 键 词： | 甘遂 肝脏 IL-β TNFα Caspase3 ALT |
| 收稿时间： | 2011/7/21 0:00:00 |
| 修稿时间： | 6/7/2012 12:00:00 AM |
Study of Euphorbia Kansui on Mouse Liver Function Correlated with Inflammatory Cytokines |
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| Lu Xi,Lei Fan,Xing Dongming,Wang Yugang,Xiao Xinyue,Hu Jun,Cheng Xianlong,Chai Yushuang,Feng Tianshi and Du Lijun.Study of Euphorbia Kansui on Mouse Liver Function Correlated with Inflammatory Cytokines[J].World Science and Technology-Modernization of Traditional Chinese Medicine,2012,14(3):1636-1641. |
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| Authors: | Lu Xi Lei Fan Xing Dongming Wang Yugang Xiao Xinyue Hu Jun Cheng Xianlong Chai Yushuang Feng Tianshi and Du Lijun |
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| Institution: | Protein Science Laboratory of the Ministry of Education, Laboratory of Pharmaceutical Sciences, School of Medicine and School of Life Sciences, Tsinghua University, Beijing 100084, China;National Institutes for Food and Drug Control, Beijing 100050, China |
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| Abstract: | This study was aimed to explore the action of ethanol extracts of Euphorbia kansui(E.KS) on mouse liver with inflammatory cytokines.By gastric administration with three dosages of E.KS(1.2,0.6,0.3 g/kg) for 2 h,4 h and 7 d,the serum was isolated for ALT.Real time PCR was employed for IL-β,TNFα and caspase 3.Histological assay was employed for the liver.The results showed that E.KS can increase serum ALT and upregulate mRNA express of IL1-β,after 2 hours of E.KS(1.2 g/kg) administration.But there was a negative action on caspase 3 expression.It was concluded that Euphorbia kansui has definite toxicity effect on mouse liver.This effect is mainly related to the inflammatory injury.The effect is not obvious related to apoptosis. |
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| Keywords: | Euphorbia kansui liver IL-β TNFα caspase3 ALT |
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