针刺对急性脑梗塞大鼠脑微循环灌注状态的影响

目的 :探讨针刺治疗脑梗塞的机理。方法 :采用血管内皮细胞荧光染色及白细胞荧光示踪法 ,结合显微录像系统和计算机图像分析系统 ,动态定量地观察了针刺对MCAo后 3、6、2 4hr软脑膜微血管形态、密度、血流速度的影响。结果 :①模型组各时段微血管内皮细胞着色差 ,组织渗出荧光多 ,针刺组明显好于模型组。②各时段模型组缺血区软脑膜微血管密度明显降低 ,针刺组微血管密度明显高于模型组。③各时段模型组缺血区软脑膜微血管血流速度降低 ,而针刺组流速较模型组明显提高。结论 :针刺能及时有效地改善MCAo后脑微循环灌注状态。

Effect of Acupuncture on Cerebral Microcirculation in Acute Cerebral Ischemia Rats

Objective: To reveal the mechanism of acupuncture in improving cerebral ischemia. Methods:A total of 120 Wistar rats were randomly divided into acupuncture (+ cerebral ischemia) group (n=36), cerebral ischemia group (n=36), sham operation group (n=36) and normal group (n=12). Cerebral ischemia was produced using middle cerebral artery occlusion (MCAo, thermocoagulation). Sixty minutes after MCAo, electroacupuncture of "Shuigou"(GV 26) and "Neiguan" (PC 6) was given to the rat by setting the parameters being frequency 4 ～10 Hz, 0.4 V, dense sparse waves and duration of 5 min. Fluorescence dyeing of the vascular endothelial cells and white blood cell fluorescein tracing were applied for observing changes of the appearance, density and blood flow velocity of the micrangia of the cerebral pia mater 3 hr, 6 hr and 24 hr after MCAo. All images were observed and recorded by microscope system of closed circuit TV and measured by computer image analyzer. Results: ① In normal group and sham operation group, the stained vascular endothelial cellular nuclei were very distinct, and the exudate staining was rather lighter. In cerebral ischemia group, the fluorescence staining of the vascular endothelial cells at different phases was lighter while that of the exudate was clearly denser, but in acupuncture group this state was obviously better; ② 3, 6 and 24 hours after MACo, the density of capillaries in the cerebral ischemic region of cerebral ischemia group decreased remarkably while that of acupuncture group was significantly higher than that of cerebral ischemia group (P<0.01). ③ 3, 6, 24 hours after MCAo, the blood flow velocity of cerebral ischemia group became very slow, while that of acupuncture group was remarkably faster than that of ischemia group (P<0.01). Conclusion: Acupuncture can reduce ischemic injury of the vascular endothelium and improve the cerebral microcirculation effectively and immediately.