温针灸对膝骨关节炎兔膝关节软骨转化生长因子β1和胰岛素生长因子Ⅰ水平的影响

目的:观察温针灸对膝骨关节炎(KOA)家兔膝关节软骨转化生长因子β1(TGF-β1)和胰岛素样生长因子Ⅰ(IGF-Ⅰ)的影响,探讨温针灸治疗KOA的作用机制。方法:30只日本大耳白兔随机分为正常组、模型组、温针灸组,采用膝关节机械制动法制备KOA模型。温针灸组取患侧内外"膝眼""阳陵泉""血海",温针灸刺激干预4周,每天1次,每次30min。4周后,采用放射免疫分析法测定各组家兔膝关节软骨TGF-β1和IGF-Ⅰ水平,HE染色后光镜下观察膝关节软骨病理形态学变化。结果:模型组兔膝关节软骨细胞TGF-β1、IGF-Ⅰ明显高于正常组(均P0.05);温针灸组膝关节软骨TGF-β1、IGF-Ⅰ表达水平较模型组显著降低(均P0.05)。HE染色结果显示,温针灸组关节软骨中软骨细胞表面较模型组光整,无簇集。结论:温针灸能通过调节KOA膝关节软骨TGF-β1和IGF-Ⅰ的水平,促进关节软骨的修复,缓解并改善膝关节局部炎性症状,起到治疗KOA的作用。

Effect of Warm-needle Moxibustion on Expression of Transfer Growth Factor-β 1 and Insulin-like Growth Factor Ⅰ in Knee Cartilage of Rabbits with Knee Osteoarthritis

Objective To observe the effect of warm-needle moxibustion on transfer growth factor-β1(TGF-β1)and insulin-like growth factorⅠ(IGF-Ⅰ)expression in knee cartilage of knee osteoarthritis(KOA)rabbits so as to explore its underlying mechanism in improving osteoarthritis.Methods Thirty Japanese white rabbits were randomly and equally divided into control,model and warm-needle moxibustion(treatment)groups(n=10in each group).The KOA model was made by immobilization of the rabbit knee.Warm-needle moxibustion was applied to internal and external Dubi(ST 35),Yanglingquan(GB 34)and Xuehai(SP 10)on the affected side for 30 min in each session and for consecutive 4weeks.The expression levels of TGF-β1and IGF-Ⅰin the knee cartilage tissue were assessed by means of radioimmunoassay.HE staining was carried out to examine the morphological changes of knee cartilage.Results In comparison with the control group,the expression levels of both TGF-β1and IGF-Ⅰ were up-regulated in the model group(P<0.05)and then down-regulated after the warm-needle moxibustion treatment(P<0.05).In addition,after the treatment the surface of the knee cartilage of rabbits became smoother and less cell proliferations and clusters were found in the cartilage in the warm-needle moxibustion group compared to those of the model group.Conclusion Warm-needle moxibustion therapy can inhibit the overexpression of TGF-β1and IGF-Ⅰin the knee cartilage of rabbits with KOA,which may be responsible for its effect in improving pathological changes of the cartilage.