电针对局灶性脑缺血再灌注大鼠神经血管单元的保护作用

目的:观察电针对大脑中动脉缺血再灌注(MCAO/R)模型大鼠神经行为学及脑组织血管内皮生长因子(VEGF)、神经生长相关蛋白-43(GAP-43)、突触囊泡蛋白(SYN)、髓鞘碱性蛋白(MBP)、勿动蛋白A(Nogo-A)表达的影响,探讨电针对MCAO/R模型大鼠神经血管单元的保护作用及其机制。方法:雄性SD大鼠随机分为假手术组、模型组和电针组,每组20只。采用线栓法制作大鼠MCAO/R模型。电针刺激在造模成功90min后进行,针刺双侧"内关""水沟""三阴交"及"百会"穴,留针30min,每天针刺1次,共14d。各组大鼠在7、14d两个时间点各取10只进行神经功能评估并行免疫组化SP法检测缺血脑组织VEGF、GAP-43、SYN、MBP、Nogo-A的表达。结果:模型组出现明显神经功能缺损症状,14d电针组大鼠神经功能恢复明显优于模型组(P<0.05)。与假手术组比较,模型组7、14d缺血组织VEGF、GAP-43、Nogo-A的表达增多,SYN在两个时间点的表达均减少(P<0.01,P<0.05);与模型组比较,电针组7、14d缺血组织VEGF、GAP-43、SYN、MBP阳性表达均增多(P<0.01,P<0.05),Nogo-A的表达减少(P<0.01)。结论:①电针能有效改善局灶性脑缺血再灌注大鼠的神经功能;②电针能通过上调各时间点局灶性脑缺血再灌注大鼠脑组织内VEGF、GAP-43、SYN、MBP的表达,下调Nogo-A的表达,促进血管、神经元、神经胶质细胞的恢复,从而保护脑缺血再灌注大鼠的神经血管单元。

Protective Effect of Electroacupuncture Intervention on Neurovascular Unit in Rats with Focal Cerebral Ischemia-reperfusion Injury

Objective To observe the effect of electroacupuncture(EA) intervention on behavior changes,expression of cerebral vascular endothelial growth factor(VEGF),nerve growth associated protein-43(GAP-43),synaptophysin(SYN),myelin basic protein(MBP),neurite outgrowth inhibitor-A(Nogo-A) in cerebral focal ischemia-reperfusion injury(CI/RI) rats,so as to study its mechanism underlying improvement of ischemic cerebral vascular disease.Methods Sixty male SD rats were randomly divided into sham-operation group,model group and electroacupuncture(EA) group.CI/RI model was established by occlusion of the middle cerebral artery(MCAO) and reperfusion.EA was applied to bilateral "Neiguan"(PC 6),"Sanyinjiao"(SP 6),"Shuigou"(GV 26) and "Baihui"(GV 20) for 30 min,once a day for 14 days.The neurologic deficits were evaluated by Longa 5-grade standard(the higher the score,the severer the neurologic deficit).The immunoactivity of cerebral VEGF,GAP-43,SYN,MBP(important in the process of myelination of nerves in the nervous system) and Nogo-A(inhibiting axonal regeneration) in the focal ischemic cerebral tissue was detected by immunohistochemistry.Results The Longa's score of the model group was significantly increased after MCAO in comparison with the sham-operation group(P<0.01).In comparison with the model group,Longa's score of the EA group was evidently lower on day 14 after CI/RI(P<0.05),suggesting an improvement of the neurological deficits after EA intervention.In comparison with the sham-operation group,the immunoactivity of cerebral VEGF,GAP-43 and Nogo-A was significantly upregulated on day 7 and 14 in the model group(P<0.01),while that of cerebral SYN was remarkably down-regulated in the model group on day 7 and 14 after CI/RI(P<0.05).Compared with the model group,cerebral VEGF,GAP-43,SYN and MBP expression levels were considerably upregulated on day 7 and 14 following CI/RI in the EA group(P<0.01,P<0.05),while that of cerebral Nogo-A was significantly decreased at the two time-points in the EA group(P<0.01).Conclusion EA intervention can effectively improve neurological function in cerebral infarction rats,which is closely related to its effects in upregulating cerebral VEGF,GAP-43,SYN and MBP expression,and down-regulating Nogo-A protein,suggesting a protective effect on neurovascular unit.