针刺干预对去卵巢大鼠血清胃饥饿素和骨密度的影响

目的:观察针刺对卵巢切除大鼠血清胃饥饿素(ghrelin)和骨密度的影响,探讨针灸治疗绝经后骨质疏松症的可能机制。方法:60只3月龄SD健康雌性大鼠,其中40只行双侧卵巢摘除术,20只做假手术,3个月后骨密度检测证实骨质疏松造模成功后各处死10只。剩余卵巢摘除大鼠随机分为模型组、针刺组、雌激素组,每组各10只。针刺组取①"关元""三阴交",②"肾俞""后三里",两组穴位交替电针治疗(连续波、2Hz、1～3mA),每次治疗20min,每日1次,连续治疗3个月。雌激素组于大鼠后腿部皮下注射苯甲酸雌二醇(0.1mg/kg),每周1次,连续3个月。应用骨密度仪测定腰椎及股骨骨密度,采用ELISA法检测血清ghrelin含量。结果:模型组大鼠血清中ghrelin的水平明显升高,骨密度值显著下降(均P<0.05);与模型组比较,针刺组和雌激素组血清中ghrelin的水平明显降低(P<0.05),同时骨密度值也有明显的提高(P<0.05);针刺组对ghrelin和骨密度的作用不及雌激素组(P<0.05)。结论:针刺干预能提高卵巢切除大鼠骨密度,下调血清ghrelin的水平,但整体作用不及雌激素。

Influence of Electroacupuncture Intervention on Serum Ghrelin Content and Bone Mineral Density in Ovariectomized Rats

Objective To observe the influence of electroacupuncture(EA) on serum ghrelin content and bone mineral density in ovariectomized(OVX) rats so as to explore its mechanism underlying improvement of postmenopausal osteoporosis.Methods Thirty ovariectomized SD rats with osteoporosis were randomized into model,EA and estrogen groups(n=10) and other 10 rats received sham operation were assigned to be control group.For OVX rats of the EA group,EA(2 Hz,1-3 mA) was applied to "Sanyinjiao"(SP 6) + "Guanyuan"(CV 4) or "Shenshu"(BL 23) + "Housanli"(ST 36),alternately for 20 min,once daily for consecutive 3 months.For rats of the estrogen group,subcutaneous injection of estradiol benzoate(0.1 mg/kg) was given once a week for 3 months.The bone mineral density(BMD) of the lumbar vertebrae and femur were detected by using a bone densitometer,and serum ghrelin content was assayed by ELISA.Results In comparison with the sham operation group,serum ghrelin level of the model group was significantly increased,and BMD of both lumbar vertebrae and femur were significantly decreased(P<0.05).Compared with the model group,the serum ghrelin levels were considerably decreased in both EA group and estrogen group,while BMD levels were significantly upregulated in the EA and estrogen groups(P<0.05).The effects of the EA group were obviously inferior to those of the estrogen group(P<0.05).Conclusion Acupuncture intervention can improve bone density and lower serum ghrelin level in OVX rats,which may contribute to its effect in improving osteoporosis.