针刺对去势大鼠骨质疏松模型雌激素及骨密度作用的研究

目的:阐明针刺对去势大鼠骨质疏松模型雌激素含量及骨密度的影响,探讨针刺治疗骨质疏松的机理。方法:将健康雌性SD大鼠32只随机分成4组:正常组、模型组、针刺组、西药组,各8只。除正常组外对其它组大鼠进行摘卵巢手术制造骨质疏松模型。针刺组采用30号针刺入双侧“足三里”“三阴交”穴,连接电针,刺激频率为1-3Hz,波形为疏密波,波宽1ms,强度0.7-1.0mA,每日1次,每次电针持续20min;西药组灌服浓度为5%的尼尔雌醇,每只灌服5mL/周,其它时间灌服等量生理盐水;正常组和模型组每日同时灌服等量生理盐水。8周后摘眼球取血,取左侧股骨。采用双能X线骨密度仪测量大鼠股骨骨密度(BMD),采用ELISA法测定血清雌二醇(E2)含量。结果:正常组、针刺组、西药组与模型组相比,BMD显著增高(P<0.01),血清E2显著增高(P<0.05),体重显著降低(P<0.01)。正常组、西药组、针刺组之间BMD及血清E2无显著性差异(P>0.05)。结论:针刺能够增加去势大鼠BMD、血清E2水平,提示这一作用是针刺治疗骨质疏松的机理之一。本实验还表明,针刺可明显降低大鼠体重,这为绝经后肥胖的针灸治疗提供了一定的实验依据。

Effect of Electroacupuncture on Plasma Estrin and Bone Mineral Density in Ovariectomized Rats

OBJECTIVE: To observe the effect of electroacupuncture (EA) on plasma estrin (E) 2 and bone mineral density (BMD) in ovariectomized (OVX) rats for studying its underlying mechanism in treating osteoporosis. METHODS: Thirty-two female SD rats were randomized into normal control, model, EA, and medication groups with 8 rats in each group. Postmenopausal osteoporosis model was established by removing the uterus under anesthesia (2% Phenobarbital, 40 mg/kg). In EA group, bilateral "Zusanli" (ST 32) and "Sanyinjiao" (SP 6) were punctured and stimulated electrically for 20 minutes with 1-3 Hz in frequency, 1 ms in duration of waves, and 0.7-1.0 mA in strength, once daily and 8 weeks altogether. Rats of medication group were drenched with 5% Nilestriol, 5 mL/week and for 8 weeks. At the end of experiments, blood samples were collected after removing the rat eyeball, and the left femoral bone tissue was taken. Serum E2 was assayed by using enzyme linked immunosorbent assay (ELISA) and BMD was measured by using double functional X-ray digital bone density meter. RESULTS: Compared with normal group, the body weight of model group was significantly bigger (P < 0.05), and that of model group was also significantly bigger than that of EA and medication groups (P < 0.11). No significant differences were found among the 4 groups before experiments and among normal control and EA groups after treatment (P > 0.05). In comparison with normal group, BMD and serum E2 of model group decreased significantly (P < 0. 01), while compared with model group, BMD and E2 of EA and medication groups increased significantly (P < 0.01, < 0.05). No significant differences were found among normal, EA and medication groups (P > 0.05). CONCLUSION: Both EA and medication can increase BMD and serum E2 in OVX rats, which may be one of the mechanisms of acupuncture in treating osteoporosis.