电针对心肌梗塞时心外膜电图和形态学的实验观察

<正> 针刺治疗冠心病心绞痛和心肌梗塞确有疗效,并能使冠心病人的缺血性心电图得到不同程度的改善。为了验证上述效应,我们采用心外膜电图和硝基四氮唑蓝(简称N-BT)染色法,观察了电针对实验性心肌梗塞的损伤范围和心肌坏死面积大小的影响。方法采用杂种健康犬,平均体重15.08±4.1公斤,雌雄不限,随机分为对照组和针刺组

EFFECTS OF ELECTROACUPUNCTURE ON EPICARDIAL ECG OF ACUTE ISCHEMIC MYOCARDIAL INJURY

The purpose of the present investigation was to demonstrate effect of acupuncture on coronary heart diseases. The mongrel dogs were anestheti- zed with pentobarbital sodium. The left anterior desending coronary artery of dog was ligated between their second and third branches. The epicardial ECG with 12 leads were recorded by means of saline-cotton-electrode. The number of leads with S-T segment elevation beyond 2 mv (N-ST) would show the extent of the ischemic damage and the sum of the S-T elevation from all 12 leads and would represent lesion. The heart was removed 2 ho- urs after ligation and then weighted. The left heart was cut into 5-6 para- llel slices and stained with nitroblue-tetrozolium (N-BT). The normal heart muscle was stained as blue colour while the necrotic area could not be stained. The infarct tissue was separated and weighed, and the perce- ntage of weight and size of left ventricle tissue was then culculated. procedure: Epicardial ECG of 12 points were recorded before the liga- tion, then the coronary arterywas tied for 120 min. At 15, 45, 60, 90 and 120 min after ligation. The epicardial ECG of the 12 points were recored respectively. In the electroacupunnure group, at 15 min after ligation, the electric square wave impulse with 2-20 Hz and 6 volts were applied on Neiguan as positive pole aid Jianshi as zero potential for 30 min. Result: 1)At 15 min ligation, the N-ST of both groups increased from 3 (before ligation) to 8.62, and the ∑ST increased from 10 (before ligati- on) to 53 mv. (P<0.01). There was no statistical difference between the two groups (P>0.5). 2) In control group, the average N-ST was 8.84 at 15 min. after l_1- gation, and increased 0.07 at 120 min. There was no statistical difference between them. In electroacupuncture group, the average N-ST was decr- eased 2.37(P=0.05). 3) In control group, the average ∑-ST was 53.04 at 15 min. after li- gation, and increased 19 mv at 120 min in electroacupuncture group the average ∑-ST was 51.19 at 15 min after ligation and decreased 11.23 mv at 120 min. (P<0.05). There was statistical significance at 120 min between the two groups (P<0.05). 4) According to the changes in ST segment of the 12 leads, it was indicated that the degree of damage was increased in control group and re- duced in electroacupuncture group. 5) The result of histological observation of myocardial infarct by N- BT staining method showed that electroacupuncture treatment could signi- gicantly decrease the extent of heart infarct. To sum up electroacupuncture treatment could save the critical margi- nal zone of the ischemic myocardium and reduce the size of infarct and the severity of myocardial damage and necrosis.