电针对大强度运动应激大鼠细胞免疫的影响

目的:观察电针对大强度运动应激大鼠T淋巴细胞亚群、脾脏指数、胸腺指数及淋巴细胞增殖能力的影响,探讨针灸防治运动性免疫抑制的机制。方法:SD大鼠随机分为空白对照组(n=10)、模型组(n=17)、电针组(n=16)。对大鼠进行为期8周、每周6次、每次150min的游泳训练,建立运动性免疫抑制模型。电针组自第2周起每次训练后在大鼠的双侧"足三里"穴、"百会"穴与"关元"穴实施电针干预。采用流式细胞仪技术测定大鼠血浆中T淋巴细胞亚群水平;分析天平称大鼠脾脏、胸腺的重量并计算脾脏指数、胸腺指数;采用3 H-TdR渗入法测定淋巴细胞增殖能力。结果:与空白组比,模型组的CD3+水平显著降低(P<0.05);与模型组比,电针组的CD3+、CD4+、NK细胞(CD161+)水平明显升高(P<0.01,P<0.05)。与空白组比,模型组的脾脏指数、胸腺指数、淋巴细胞增殖能力均显著性下降(P<0.01,P<0.05);电针组脾脏指数、胸腺指数明显高于模型组(P<0.01)。结论:长期剧烈的运动应激导致大鼠的免疫功能下降,甚至抑制,但电针能明显提高CD3+、CD4+、NK细胞的水平,同时能有效防止大鼠脾脏指数、胸腺指数、淋巴细胞增殖能力的显著性下降,提示电针对运动性免疫抑制具有正向调节作用。

Effects of electroacupuncture on T-lymphocytes, spleen index, thymus index and lymphopoiesis levels in strenuous exercise-induced stress rats

Objective To observe the effect of electroacupuncture(EA) on levels of T-lymphocytes,spleen index,thymus index and lymphopoiesis in strenuous exercise-induced immunosuppression rats,so as to study its mechanisms underlying improving immune function of strenuous training athletes.Methods A total of 43 SD rats were randomly allocated to control(n=10),model(n=17) and EA(n=16) groups.Immunosuppression model was established by forcing the rat to swim for 150 min in a water tank,once daily,6 times a week and for 8 weeks.EA(2 Hz,<1 mA) was applied to bilateral "Zusanli"(ST 36),"Baihui"(GV 20) and "Guanyuan"(CV 4) for 20 min,once daily for 7 weeks.Spleen weight and thymus weight were detected after killing the anesthetized rats for calculating spleen index and thymus index,separately.Peripheral blood T cell subsets were mea-sured by using flow cytometer,and lymphopoiesis was detected by using 3H-thymidine deoxyribonucleotide(3H-TdR) incorporation.Results In comparison with the control group,spleen index,thymus index,lymphopoiesis and CD3+ levels of the model group were decreased significantly(P<0.01,P<0.05).Compared with the model group,peripheral blood CD3+,CD4+ and NKC levels,and thymus and spleen indexes were increased considerably in EA group(P<0.01,P<0.05).No signi-ficant differences were found between the control and model groups in CD4+,CD8+and CD4+/CD8+and NKC levels,and between the model and EA groups in CD8+and CD4+/CD8+ levels(P>0.05).Conclusion EA intervention can effectively upregulate peripheral blood CD3+,CD4+and NKC,and thymus and spleen indexes in strenuous training rats,which may contribute to its effect in relieving strenuous exercise-induced immunosuppression.