快速老化痴呆小鼠海马、皮层p130表达的增龄变化及针刺的影响

目的:观察针刺对快速老化痴呆小鼠(SAMP10,简称P10)脑中p130表达的影响,探讨针刺治疗痴呆的可能机制。方法:随机将P10分为对照组、针刺组、非穴组,同源正常老化小鼠(SAMR1,简称R1)为正常对照。针刺组采用"益气调血,扶本培元"针法(简称"气血"针法),选穴"膻中"中脘"气海"、双侧"足三里"、双侧"血海"。"血海"用捻转泻法,余穴施捻转补法,每穴30s,每天1次,第7天暂停,连续13d。运用western blotting技术,观察2、4、6、8、10、12月龄P10海马和皮层中p130表达的增龄变化,以及"气血"针法对8月龄P10海马和皮层p130表达的影响。结果:①p130表达在正常老化R1海马和皮层均随增龄呈平稳上升趋势,6月龄后与2月龄存在明显差异(P<0.05,0.01)。P10则呈波动下降趋势,海马中4月龄后表达即下降,6月龄达谷值(P<0.01),6至8月龄回升,8月龄低于2月龄水平(P<0.05),8至12月龄又下降;皮层中2至4月龄下降,4月龄后开始上升,至6月龄达峰值(P<0.01),6至8月龄下降至低谷,8至12月龄又上升,但仍停留在较低水平。②与8月龄R1相比,p130在P10海马和皮层表达分别呈上调与下调。"气血"针法具有腧穴特异性,可良性调节皮层的蛋白表达,使之接近R1水平。结论:p130表达在R1与P10同脑区间随增龄存在差异,且p130的波动期与报道的P10生物学特征出现的时间段接近。"气血"针法可良性调节8月龄P10皮层p130的表达。

Age-related Changes of P 130 Expression in Hippocampus and Cerebral Cortex and Effects of Acupuncture in SAMP 10

OBJECTIVE: To observe the effect of acupuncture on p130 expression in neocortex and hippocampus of Senescence Accelerated Mouse/Prone (SAMP) 10 so as to explore its underlying molecular mechanism in treating dementia and aging-related diseases. METHODS: Thirty SAMP 10 were randomly divided into model, acupuncture, and non-acupoint groups with 10 cases in each, and 10 SAM/Resistant (SAMR)1 were used as normal control group. The needle manipulation of "Yi Qi Tiao Xue, Fu Ben Pei Yuan" (Reinforcing qi and blood, and strengthening the body resistance) was applied to "Shanzhong" (CV 17), "Zhongwan" (CV 12), "Qihai" (CV 16), "Xuehai" (SP 10) and "Zusanli" (ST 36), once daily for two weeks. p130 expression of neocortex and hippocampus was assayed by western blotting technique. RESULTS: In SAMR 1 control group, p 130 expression in the neocortex and hippocampus exhibited an age-related stable upregulation from month 2 to 12, being significantly from the 6= month (neocortex) on and 8th month (hippocampus) on respectively (P < 0.05, 0.01). In SAMP 10 group, hippocampal and neocortical p130 expression exhibited an age-related downregulation change in the same period, fluctuating in about 6th month (downregulated remarkably in hippocampus, P < 0.01; upregulated markedly in neocortex, P < 0.01). In comparison with SAMR 1 (8 months old) group, p130 expression in hippocampus in SAMP 10 model group was upregulated significantly (P < 0.05); while that in neocortex downregulated considerably (P < 0.05). The expression of p 130 in neocortex in acupoint-acupuncture group was significantly higher than that in SAMP 10 model and non-acupoint acupuncture groups (P < 0.05). No significant differences were found between acupoint- and non-acupoint acupuncture groups in hippocampal p 130 expression, and between non-acupoint acupuncture and SAMP 10 model groups in neocortical p 130 expression (P > 0.05). CONCLUSION: Along with the increase of aging, p 130 expression in both hippocampus and neocortex increases gradually in SAMR 1, while decreases in SAMP 10, in spite of fluctuating at month 6 in age. Acupuncture can beneficially regulate the aberrant expression of p130 in neocortex of 8-month-old SAMP 10.