电针远近部位穴位对暂时性脑缺血大鼠脑组织、血清一氧化氮含量影响的比较研究

目的 :比较不同部位穴位对实验性脑缺血疗效的影响。方法 :采用大鼠大脑中动脉暂时性缺血模型 ,比较电针头部的“百会”、“水沟”穴和远离头部的“环跳”、“昆仑”穴 ,对脑组织、血清NO含量的影响。结果 :缺血模型组与假缺血组比脑组织、血清NO含量显著增加 (P <0 .0 5) ;与缺血模型组比较 ,头部穴位组明显降低 (P <0 .0 1 ) ,肢体部穴位组降低无显著性意义 (P >0 .0 5) ;两穴位组之间比较 ,脑组织NO含量无显著性差异 ,血清NO含量有显著性差异 (P <0 .0 1 )。结论 :尚不能认为远近部位穴位的作用有差别

Comparative Research on the Influences of Electroacupuncture of Adjacent and Distant Acupoints on NO Contents of Brain Tissues and Serum in Rats with Temporary Cerebral Ischemia

Objective: To compare the curative effects of electroacupuncture (EA) of different acupoints on experimental cerebral ischemia. Methods: 41 SD rats were randomly divided into sham ischemia group (n=9), ischemia group (n=10), head acupoint group (n=11) and limb acupoint group (n=11). A rat model of temporary ischemia of middle cerebral arterial was used to compare the influences of EA of head acupoints "Baihui"(DU 20) and "Shuigou"(DU 26) and distant acupoints "Huantiao"(GB 30) and "Kunlun"(BL 60) on the NO contents of brain tissues and serum. The parameters of EA were frequency of 4～ 20 Hz, electric current of 3 mA, sparse dense waves and stimulation duration of 45 min. The serum and brain NO contents were detected with nitrate reductase method. Results: The NO contents of brain tissues and serum in ischemia group were significantly higher than those of sham ischemia group (P<0.05) and head acupoint group (P<0.01); but there were no significant differences between head acupoint group or limb acupoint group and sham ischemia group, or between head acupoint group and limb acupoint group in brain tissue NO contents; or between head acupoint group and sham ischemia group or limb acupoint group in serum NO contnts (P>0.05). Conclusion: EA of both head acupoints and limb acupoints can effectively lower cerebral ischemia reperfusion induced increase of NO level to reduce ischemic injury of the cerebral tissues. The results of the present study can not lead to a conclusion of existence of difference between EA of adjacent and distant acupoints in relieving cerebral ischema.