针刺对高血脂合并脑缺血大鼠海马区Bcl-2、Bax蛋白表达的影响

目的:探讨针刺对高血脂合并脑缺血损伤大鼠海马区Bcl-2、Bax蛋白表达的影响。方法:雄性SD大鼠,随机分为正常对照组、脑缺血模型组、高血脂合并脑缺血模型组、高血脂合并脑缺血治疗组,每组10只。采用经典高脂配方饲料喂养并结合化学刺激诱导血栓性闭塞大脑中动脉造成大鼠局灶性脑缺血联合模型。高血脂模型造成后先电针“三阴交”“丰隆”,每日1次,治疗10 d,然后再造脑缺血模型,同时针刺“百会”“水沟”,及电针“三阴交”“丰隆”,每日1次,共治疗7 d。治疗结束后,应用免疫组化方法观察针刺前后大鼠海马区Bcl-2、Bax蛋白表达的变化。结果:大鼠局灶性脑缺血后Bcl-2表达降低,Bax表达升高,Bcl-2/Bax比值下降(均P<0.01);针刺后脑缺血大鼠Bcl-2表达增加(P<0.05),Bax表达降低(P<0.01),Bcl-2/Bax比值升高(P<0.01)。结论:针刺能增强Bcl-2的表达,抑制Bax的表达,这可能是针刺减轻脑缺血损伤的机制之一。

Effect of Electroacupuncture on Protein Bcl-2 and Bax Expression in Hippocampus in Hyperlipemia Rats with Concurrent Cerebral Ischemia

Objective: To observe the effect of electroacupuncture(EA) on protein Bcl-2 and Bax expression in hippocampus in hyperlipemia rats with concomitant cerebral ischemia(CI).Methods: Forty male SD rats were randomized into control,CI,hyperlipemia CI,and hyperlipemia CI EA groups,with 10 cases in each group.Hyperlipemia model was established by feeding the animals with high fat forage for 6 weeks and CI model established by occlusion of the unilateral middle cerebral artery.EA(1-(3 mA),(15 Hz)) was applied to bilateral "Sanyinjiao"(SP 6) and "Fenglong"(ST 40) for (20 min) every time;and "Baihui"(GV 20) and "Shuigou"(GV 26) were punctured and stimulated by twirled the acupuncture needle with hand continuously for(1 min).The treatment was conducted once daily and continuously for 17 days. The expression of protein Bcl-2 and Bax in the hippocampus was displayed by using immunohistochemical method.Results: In comparison with normal control group,Bcl-2 immuno-reaction(IR) positive cells in the hippocampus in CI and hyperlipemia CI groups decreased significantly(P<0.01),Bax IR positive cells in the same two groups increased significantly(P<0.01),while Bcl-2/Bax also reduced remarkably(P<0.01),meaning that after ischemia the expression of Bcl-2 was downregulated and Bax upregulated markedly.In comparison with hyperlipemia CI group,Bcl-2 IR activity increased,Bax IR activity reduced and Bcl-2/Bax increased considerably in hyperlipemia CI EA group(P<0.01,0.05),suggesting that EA could reverse the effects of hyperlipemia and CI.Conclusion: EA can eliminate hyperlipemia-and cerebral ischemia-induced abnormal expression of Bcl-2 and Bax in hyperlipemia rats with concomitant cerebral ischemia.