电针“关元”、“三阴交”对围绝经期模型大鼠神经内分泌的调整作用

目的 :研究电针“关元”、“三阴交”对围绝经期模型大鼠神经内分泌的调整作用。方法 :复制大鼠去卵巢模型 ,测定电针“关元”、“三阴交”对模型大鼠血清雌激素 (E2 )、促卵泡激素 (FSH)、黄体生成素 (LH)和下丘脑 β EP含量的影响。结果 :模型组较正常组血清E2 含量降低 ( 2 2 77±0 1 3 5ng/L) ,FSH、LH含量升高 ( 7 0 6 2± 1 3 56mIU/mL ,3 473± 0 3 55mIU/mL) ,下丘脑 β EP含量显著降低 ( 1 6 751± 1 484pg/mg) ;电针组较模型组血清E2 含量显著提高 ( 3 44 9± 0 1 53ng/L) ,FSH、LH含量显著降低 ( 5 71 4± 0 3 50mIU/mL ,2 6 3 8± 0 1 98mIU/mL) ,下丘脑 β EP含量显著升高 ( 2 2 1 2 2± 0 82 8pg/mg组织 )。结论 :电针“关元”、“三阴交”治疗围绝经期综合征通过调节生殖内分泌和调整自主神经功能紊乱发挥作用

Effects of Electroacupuncture of "Guanyuan" and "Sanyinjiao" on Neuroendocrine Function in Perimenopause Model Rats

Objective: To investigate the effects of electroacupuncture (EA) of "Guanyuan" (CV 4) and "Sanyinjiao"(SP 6) on neuroendocrine function in perimenopause model rats.Methods:Rat perimenopause model was made by removing the ovarium. 40 SD female rats were randomly divided into control group, EA group, model group and estradiol group, with 10 cases in each group. Serum estrin (E)2, follicle stimulating hormone (FSH), luteotropic hormone (LH) and β-EP of the hypothalamus were determined with radioimmunoassay before and after EA of SP-6 and CV-4 (2～100 Hz, 2.5 V, 20 min/time, once daily, 10 days/therapeutic course). In estradiol group, muscular injection of estradiol (0.05 μg/10 g) was given to the rats, once every other day. Results:In comparison with control group, serum E 2 and hypothalamic β-EP of model group decreased significantly (P<0.05), serum LH and FSH of model group increased markedly (P<0.05). Compared with model group, serum E 2 and hypothalamic β-EP of EA group and estradiol group increased apparently (P<0.05), while LH and FSH of these two groups lowered remarkably (P<0.05). Findings indicated that EA treatment could significantly raise serum E 2 and hypothalamic β-EP, and obviously lower serum LH and FSH levels. Conclusion:Electroacupuncture of "Guanyuan" (CV 4) and "Sanyinjiao"(SP 6) can regulate neuroendocrine function in ovariectomized rats, suggesting EA may play a role in improving menopause syndrome.